Why are functional gene results (nosZ, amoA) compared to 16S gene?

I am working on the effect of pH on functional genes in the nitrogen cycle (amoA, nosZ..). Some of the papers i have read present results based on the ratio between functional gene (like nosZ) and...

27 December 2020 1,625 6 View

Why would gel electrophoresis need to be run for 16 hours at 2-8 deg C? Is there an alternative protocol that takes less time or works at room temp?

I am trying to separate and analyze macromolecules (DNA) using horizontal gel electrophoresis. Protocol involves 1X TAE buffer, 10X denaturing buffer, 1X running buffer, 1% agarose gel, and 2X...

18 November 2020 667 2 View

Any tips and tricks to prevent bubbles from being trapped in polymer film during membrane formation via immersion precipitation?

05 July 2017 6,398 6 View

Does anyone know a low molecular weight agarose gel?

I need a trade name for any low molecular weight agarose gel that can dissolve in water. My cDNA is giving me my band of interest (which is faint) and a smear on gel and I want to cut out my band...

23 April 2014 8,122 2 View

Why does the level of phenobarbital spiked in blood appear to be lower than the actual spiked amount when being screened by ELISA?

5ppb of phenobarbital have been spiked into blank blood. This have been replicated by 10 times. However, ELISA results show that all 10 of the replicates contains LESS than 5ppb (average of...

14 July 2013 963 1 View

Can anyone help me with a home made OGD?

I'm hoping to set up some oxygen glucose deprivation experiments using cultured cells but only have a CO2/air incubator. I was wondering whether there was any way of home rigging a small nitrogen...

06 June 2013 2,650 2 View

Will the formation of semicarbazone from an unknown (suspected to be a ketone) be long, or has it been falsely analysed?

In an undergraduate organic experiment, we are required to identify the functional group present via solubility tests (with H2O, HCl, NaOH and NaHCO3) and Classification Tests (Brady's, Tollen's,...

10 March 2013 3,007 6 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 8,920 1 View

Could you please suggest to me an interesting and good book about Nanotechnology?

I want to know about nanotechnology from beginning to professional. Thus I want some book in this area

01 February 2021 1,889 13 View

How to find statistical data of EEG signals?

Hi all, I hope everyone is doing good. I am working on Machine Learning, I am working on EEG data for which I have to extract statistical features of the data. Using mne library I have extracted...

29 January 2021 6,686 1 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View