Dear Sir. Concerning your issue about the limits (CFU/ml) for a pathogens isolated from wastewater. The microbial counts, characteristics and frequency of occurrences of the isolated microorganisms over a given period of time were determined by cultural, morphological and biochemical characteristics using established procedure while the varying concentrations. I think the following below links and attached files may help you in your analysis:

https://pubag.nal.usda.gov/pubag/downloadPDF.xhtml?id=55092&content=PDF

http://www.umass.edu/mwwp/pdf/sm9010-40intro.PDF

http://aem.asm.org/content/78/22/8089.full

http://cest2015.gnest.org/papers/cest2015_00238_poster_paper.pdf

Thanks

No limitation for pathogens count in wastewater, it depends on microbial consortia present in the wastewater. the isolation of the pathogen from wastewater may have high violent than freshwater pathogen, it may give the idea to develop the new disinfectant (valuable)

yes, there is no limit regarding number or types of pathogens in waste water.

there is no limits (CFU/ml) for pathogens isolated from wastewater ....

Dear Dr Hussein,

E.coli and Clostridium perfringens are the most common “indicator” organisms. They indicate that domestic sewage may be present and therefore a risk of pathogens (eg salmonella, giardia, helminths etc).

cfu limits are valuable in conjunction with other management controls to assess and minimise health risks in effluent recycling applications. For example, use of effluent for watering food crops eaten raw should have

Isam Eldin Hussein Elgailani anwer link are very important

Article Pathogens in Water: Value and Limits of Correlation with Mic...

There is no limited value

PI take alook at this RG link

https://www.researchgate.net/publication/263779119_Contamination_of_water_resources_by_pathogenic_bacteria

yes, there are standard numbers of CFU in every type of water, drinking water, beach water, wastewater, etc....

https://www.who.int/bulletin/archives/78(9)1104.pdf

Article A Novel Approach for Pathogen Reduction in Wastewater Treatment