Hello,

I do not know any paper on Lepidium sp. in which authors used SCoT markers. However, this is a universal method for DNA fingerprinting (applied so far on many plant species) so I am sure that you can use it in your studies.

Best wishes

Piotr

Dear Dr. Androsiuk

Thank you for your interest. Do you think the primers used in other plants can also be used for this genus?

Hi,

I think that at the beginning you should have a look on another papers where the SCoT primers were used and pick those which were used the most often. I tried the same scheme for my preliminary studies with Avena sp. and Poa sp. and it worked. I ordered 20 SCoT primers and got amplifcation for all of them but of different quality and various number of bands (amplification products). However, as you know, there is no rule for that, but you should also try them in your studies

Good luck

Piotr

Hi once again,

in my last post I forget to ask you whether you are considering using another molecular markers in your study on Lepidium? I can recommend you retrotransposon based system called iPBS by Kalendar et al. 2010.

DOI: 10.1007/s00122-010-1398-2

It is also universal method of genotyping associated with resolving of amplification products on agarose.

According to my opinion the iPBS markers give better DNA profiles on gels (higher number of bands, more clear, easy to score) in comparison to SCoT markers. If you are still at the stage of planning your experiment you could consider using this marker system.

Best wishes

Piotr

Hi

Thank you for your suggestion. The project has been approved, and in addition to SCoT, I use SSR and ISSR markers to study the genetic diversity of the accessions of three Lepidium species.

You can google search it or can search in pubmed central. SCot marker can be used for any plants and animals. It is based on Start codon region of a gene. There are many different primers available which u can use for the study.