Discrepancy protein levels and receptor expression?

I measured the expression of a protein that is a receptor in a panel of cell lines. When I measured the protein levels by Western blot the protein levels were about the same in all cell lines ,...

09 September 2020 6,671 3 View

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

02 February 2020 4,585 2 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

27 February 2018 1,151 2 View

How to calculate the surface coverage of two adjacent but no overlapping cellular structures?

Dear all, I am staining two adjacent cellular structures solids with fluorescent antibodies (Alexa-488 and 555). I want to calculate the coverage of the small solid respect to the big one. In...

12 February 2018 2,606 3 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

01 February 2018 4,079 2 View

Thermal capacity in compact model of RRAM: fit parameter or physical constant?

The thermal capacity in compact model of RRAM: should be an adjustment parameter or a physical constant dependent on the transition oxide and upper and lower metals. What do you think about this?

06 May 2017 8,995 1 View

Thermal capacity in compact model of RRAM: fit parameter or physical constant?

The thermal capacity in compact model of RRAM: should be an adjustment parameter or a physical constant dependent on the transition oxide and upper and lower metals. What do you think about this?

04 May 2017 7,680 1 View

No title

26 February 2017 4,268 6 View

What's the meaning of anti-disolvent?

suspension of particles in a non-aqueous anti-solvent

01 February 2017 4,805 0 View

Where can I find mean pairwise relatedness coefficients for MULE DEER (Odocoileus hemionus)?

We did a study on a free-ranging population of this species, in which we tested whether spatial associations (2 adult deer within 25 m of each other) varied with several factors such as season,...

20 April 2016 165 4 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View