We measure hormones in animals in the same method that we measure for humans. But do reference values differ or not?
The reference values is altered form area to area and changes according to genetic variation and spp as well as to hybridization
So let me know what your type of rabbits and purity
the hormonal reference values need specified for rabbit to match with ordinary values, but you can use placebo as a reference with treated group comparison or can overcome by make dilution factor with body volume or body weight.
A reference value reflects what is used as the "standard" in an assay. For steroid hormones like progesterone or estradiol-17B, the standard is the same among many species. For protein hormones like prolactin or follicle-stimulating hormone, the proteins differ among species and their cross-reactivities are not 100%. It is important to choose a standard hormone that is as much as possible identical to the natural hormone produced in the species of interest. Nevertheless, if there is not a reliable source of a hormone for that species, chose one that is as closely related as possible. MOST IMPORTANTLY, a reliable hormone standard should show a parallel assay line with dilutions of blood from the species of interest within the same range of activity in the standard curve. If the lines are not parallel, then the standard may not be reliable for that species.
Having done a number of studies on sex hormones in laboratory animals like rabbits and rats, i observed that reference values in these animals differ slightly from that of humans. My findings are FSH (4.0 -12.0 miu/ml), LH (3.0 - 9.0 miu/ml), Estrogen (10.0 - 20.0 ng/ml), Testosterone (1.5 - 4.0 ng/ml).