Are there recommendations for shRNA cloning protocols?

28 March 2017 1 4K Report

I want to synthesize dsDNA coding for shRNAs from two complimentary oligos, anneal them and clone it into a retroviral vector. Due to hairpin formation within the two oligos, I predict the annealing of the oligos to each other to be challenging. Are there any good protocols that are available? This is my first shRNA design and cloning. Any help appreciated.

Shubha Suresh

Dear Katharina,

This looks like a great resource. Thank you!

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