We seek recommendations for methodology but not with commercial detectors of the ambient air sort. Any real use recommendations ? GC methods could be one approach if I build it in the project.
Some things Ive found:
ASTM Methods for Measurement of H2S are largely geared for the petroleum industry in the list from Moore and Spitler.
Standard Test Method for Hydrogen Sulfide in Liquefied Petroleum (LP) Gases (Lead Acetate Method)
Standard Test Method for Analysis of Hydrogen Sulfide in Gaseous Fuels (Lead Acetate Reaction Rate Method)
Standard Test Method for Hydrogen Sulfide in the Atmosphere by Rate of Change of Reflectance
Standard Practice for Determining Concentration of Hydrogen Sulfide by Direct Reading Length of Stain, Visual Chemical Detectors
Standard Test Method for Qualitative Analysis for Active Sulfur Species in Fuels and Solvents (Doctor Test)
Standard Test Method for Determination of Sulfur Compounds in Natural Gas and Gaseous Fuels by Gas Chromatography and Chemiluminescence
Standard Test Method for Determination of Sulfur Compounds in Natural Gas and Gaseous Fuels by Gas Chromatography and Flame Photometric Detection
-seeking methods to quantify H2S at PPB levels in biologic samples (but not in ambient air) ...any user suggestions?. Available from: https://www.researchgate.net/post/seeking_methods_to_quantify_H2S_at_PPB_levels_in_biologic_samples_but_not_in_ambient_air_any_user_suggestions [accessed Apr 7, 2017].
It is not an easy task to measure just hydrogen sulfide in "biological sample", because as a biochemical molecule, hydrogen sulfide can occur (depending on ionic strength and pH) in molecular configuration as sulfide ion or as dissolved gas. As a rule assume that in the environment at a pH above 8.5, hydrogen sulfide is mostly present as sulfide ion and at pH below 8.5, hydrogen sulfide is mostly present as dissolved gas. Of course at any pH below or above pH 8.5 there will be different equilibrium between sulfide ion/hydrogen sulfide dissolved gas. Your approach to measurements of hydrogen sulfide will depend very much on the kind of "biological sample", for instance tissue, blood, urine, fecal matter etc. .
Thanks,
I need to try an will be e=developing my own system to couple with a gas instrument then.
THanks
Yes, it is possible.
Please contact with my colleague Dr Mard: [email protected]
Best regards
There is a lead acetate method, if anyone uses this let me know.
We prefer to use the release of volatile organic compounds by GC and the headspace of the GC. Blood, Urine and stool are the particular biologic matrices at this time. We looked at breath, but this as not reliable as a source.
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