How to extract nuclei from OCT frozen tissue?

I am trying to extract intact nuclei from OCT-frozen tumor tissue samples. Does anybody have a protocol for doing so? I need as many intact nuclei as possible for performing ATAC-seq on them.

13 September 2020 8,608 1 View

Proteins getting stuck at thr interface of stacking and resolving gel of a 6M urea gel, Why?

I am trying to separate a 40 & 43 kda protein. I run 6M urea sds page for that. But most of the proteins get stuck around the interface of resoving and stacking gel. The small amount of...

19 June 2018 3,189 4 View

There is a question about western blotting in huntington model. emergency?

i created a huntington's disease model using plasmide gfp-q74 and gfp-q23(from addgene) in SHSY5Y cell line. then i made BCA for quantification of huntingtin protein and then i made western...

23 March 2018 5,760 3 View

How to study the levels of Phosphorylated Tight junction proteins like ZO1 and Occludin?

Hi all, I want to check the expression of phophorylated ZO1 and occludin from cell-lysate. However I couldn't get any Antibody commercially for phospho-ZO1 or Phospho-occludin as available for...

07 March 2018 7,984 3 View

Singlet to triplet/quintet tddft?

I have tried to do tddft from singlet to quintet state. by writing "#p TD(NSTATE=20,quintet) pbe1pbe/def2svp scf=(xqc,maxcycles=9000)". and have mentioned spin multiplicity=1, but it's shows...

30 January 2018 8,117 1 View

Why there are differences in calculation of Refractive Index of nanomaterial , when calculated from 2 route, from reflactance and transmittance data?

please give theoretical analysis

12 April 2017 1,940 2 View

Hi guys, How can I calculate Relative Fluorescence Intensity (RFI) with flowcytometry?

22 September 2016 3,189 3 View

Why does sometimes the photo-current in an photo-electrode keep increasing instead of decreasing on illumination?

When an electrode made of semiconductor nanotubes/nanowires etc is illuminated an overshoot or transient maximum in the photocurrent is observed. The photocurrent gradually exponentially decreases...

11 September 2016 6,372 1 View

I have excessive mesh distortion. What can be done?

01 February 2016 2,360 4 View

Can anyone please tell me the redox potential Eo,redox of aqueous sodium sulfate and aqueous RhB solution?

11 January 2016 3,459 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View