At the current moment I've come across 2 studies: Castelino et al. 2017 and Parnell et al. 2017 who compared different regions regarding amplification of low biomass samples and the comparison with negative controls (Parnell). Perhaps anyone could suggest any other study covering this topic?
Muhammad Arslan: Yes, the exact question is not clearly understood
Hi Davis! What is your biological question? I think these studies you mentioned already enough to understand the selection of different hypervariable regions for diversity analysis. Maybe if you put your question directly, people able to answer that questions specifically.
Thank you for your answer Muhammad Arslan : the idea behind the question would be - Are there any other study you would recommend reading that also cover this topic besides Pernell and Castelino?
I guess this is more of a question for discussion not clearly a biological question, sorry for the misunderstanding.
Hello Davis, Kerrigan et al., 2019 have amplified both the V4 and V6 hypervariable regions of Seawater and Marine Sediment for the microbiome analysis. You can check it at https://www.frontiersin.org/articles/10.3389/fmicb.2019.01640/full
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