Are there any strains of E. coli that do not produce IAA?

07 August 2024 0 3K Report

Hello! I'm looking for any strains of E. coli or other commonly-used model bacteria that have been confirmed to produce NO indole-3-acetic acid. I am quantifying IAA production on several bacteria of interest, and I would like to have a negative control besides the un-inoculated medium itself. Thank you for your help!

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

More Kieran Szelong's questions See All

How to calculate mass of reagents based on desired pH and molarity?

Dear all, I am looking for guidance relating to a formula to calculate mass of reagents based on the desired pH and molarity. Is there a formula for this? I am looking to create, and justify with...

28 March 2024 7,457 1 View

THLE-2 Media Alternatives?

Hi All, Has anyone had any success culturing THLE-2 cells in media other than the suggested BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit. I have found a couple of references where...

23 August 2023 1,851 0 View

Should Bonferroni Correction be applied in this scenario (multiple DVs)?

Hi Everyone! So I'm conducting research whereby there is a 2x6 within subjects repeated measures design (2 modes of exercise and 6 intensity levels). I have conducted pre-planned comparisons...

13 May 2023 4,349 2 View

To what extent do motorhomes and other large vehicles degrade road surfaces in relation to cars and other light vehicles?

I am studying the effects on the road system of a rural region of a drastic increase in traffic, particularly Motorhomes. My question is this, do motorhomes and other large vehicles have a...

22 October 2020 8,471 3 View

Question regarding researchers of same name?

I hope somebody may be able to help me - I have just registered with this website, Research Gate, but there is another Kieran O'Driscoll here, some of whose articles in the hard sciences are for...

02 May 2019 3,542 3 View

Interviewing members of the public to determine public perception of borderline personality disorder? What questions should i ask?

Just some context - Ive read many research reports to try and determine an appropriate set of questions for my interviews but to no avail. Each set i have made however keeps getting knocked back...

24 January 2019 9,936 3 View

Can anyone recommend a validated tool to assess the appropriateness of antipsychotic prescribing in long term care residents with dementia?

I have come across two different tools in my preliminary searching 1) The quality use of medications in dementia (QUM-D) tool by Peisah et al. 2) The Appropriate Psychotropic drugs use In Dementia...

06 June 2017 9,036 6 View

Would Sulpiride be considered a typical or atypical antipsychotic?

There is conflicting information between various sources as to whether is the former or latter.

08 July 2015 8,856 11 View

Can results of food diaries and 24 hour recalls be used to measure nutritional intake for different participants in the same study?

For a research study I'm planning on conducting, we want to give participants the option of whether to provide information about their nutritional intake either via telephone, in a written diary...

26 March 2014 6,781 26 View

Does anyone know of any systematic reviews that look at the cost and value of selection procedures for medical school?

A programmatic approach to assessment is likely to be best practice, but it is expensive. I wonder is there any evidence?

24 March 2014 8,332 3 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

What is the method of standard curve of IAA preparation?

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...

29 June 2024 4,421 1 View

Which medium is the best to be used for qualitative estimation of IAA production of PGPR?

As per the tiltle said, I want to qualitatively test how much IAA can be produced by some of the bacteria that I have just isolated from a soil sample. After culturing the soil bacteria in a...

03 December 2023 4,408 1 View

Why did I get opacity and then precipitation using Salkowski reagent?

I used Salkowski reagent 2% 0.5 M FeCl3 in 35% perchloric acid to quantify IAA. 1 ML of bacterial culture (very clean) supernatant was mixed with 1 mL Salkowski reagent, the mixture become opaque...

23 November 2022 926 2 View

What concentration of hormone eg., IAA, Gibbrellin, ABA, Jasmonic acid in hydroponic condition? please suggest me ?

Dear researchers, Please suggest me how much concentration of these hormone (IAA, ABA, Gibberelic acid, jasmonic acid, ) for Expression Analysis of some genes.

13 October 2022 9,374 1 View

Are there cheaper alternatives to C18 used in GA (gibberellic acid )and IAA( indole acetic acid) analysis?

Are there cheaper alternatives to be used during analysis of GA (gibberellic acid) and IAA(indole acetic acid) #gibberellicacid #Auxin

31 July 2022 7,491 0 View

Why transformed melon explant always undergo necrosis and how to get shoots from the callus?

I have been trying to do regeneration of rockmelon plant using cotyledonary explant. After a few years of trying, I'm not getting the shoots. I'm able to get up to the callus stage but the callus...

24 July 2022 267 0 View

Struggling for rooting of my shoot induced callus of Astro myriostigma. Till shooting it works but could not get root> can anyone offer advice?

Tried 0.5-1 mg/ml IAA for rooting but no roots

21 July 2022 2,961 0 View

Why the Erlich or Salkowski reagent doesn´t dissolve and neither generate color when added to the IAA concentrations?

Standard calibration curve for IAA quantification by spectrophotometric method Could you be so kind to explain me why the Erlich or Salkowski reagent doesn´t dissolve and neither generate color...

25 May 2022 8,342 0 View

Growth hormones estimation?

What are the growth hormonal parameters that can be estimated from endophytic fungi except for auxin (IAA)?? Please also mention their colorimetric protocol for estimation or quantification.

10 April 2022 1,900 3 View