Dear Venkata:

Freezing damages mitochondrial functions irreversibly because of the damage of the inner membrane. However, for some purposes you can use those mitochndria. To prepare submitochondrial particles, for example. To isolate mitochondria is relatively easy procedure. On my Research Gate website you can find my book "Practical mitochondriology" with the methods described.

Sincerely,

Al;exander Panov

Freeze-thawing cycles progressively damages mitochondria from whatever source. I know of postmortem assessment of organ mitochondrial viability, whose reports submit that membrane potential had dropped. However to isolate mitochondria from yeast, animal, plant is not difficult, the underlying principle are homogenisation with or without enzyme digestion, differential centrifugation using suitable buffers. Ideally, mitochondria are meant to be used fresh, or within 4 hours of isolation.

Dear Alexander V Panov and Ebenezer idowu Ajayi...... Thank you very much for your answers.