My question stems from significant differences in bio-analytical data of a small molecule, when samples were diluted using SCID plasma (high values) v/s CD1 plasma (very low values).
We inherited an old incubator and it showed mould growth in the water tray, so we used an internal service for formalin fumigation (and formalin deactivation with ammonia). They did everything to...
20 November 2022 5,112 3 View
I have prepared carbon nanodots by using natural precursor as carbon sources. now for the determination of concentration of prepared soluble carbon nanodots what can I do? as earlier mentioned in...
01 July 2021 1,431 1 View
In my previous understanding, E. gracilis accumulates paramylon when grown in high-carbon medium, especially if grown in the dark. I have reproduced this in the past using KH medium. However, I...
16 April 2021 260 1 View
I have synthesis Carbon nanodots by using water as a solvent. now facing so much problems during the analysis ( spectroscopic techniques)
16 March 2021 9,548 3 View
The relationships between Science, Beliefs and Faith have always generated a heated debate (which has reached popular opinion) leading to think that they are irreconcilable concepts and that a...
26 October 2020 2,755 99 View
As we know, not long ago the WHO named Wuhann Pneumonia (SARS-COV 2): Covid-19 (from Coronavirus Disease 2019), which has become a serious pandemic and health emergency with multiple pathological...
21 September 2020 4,870 13 View
Apparently there is a belief that contaminated steam in the autoclave can contaminate your pipette tips while sterilizing them with biomolecules such as peptides and DNA, and that this can be a...
05 March 2020 1,660 3 View
Sexual appeals is normally used by brands to catch the attention of the viewers but for fashion brands like Calvin Klein and Dolce & Gabbana, the brand recall value is anyway high. Then why do...
08 April 2018 327 8 View
Namaskar I am working on panel data. As per my regression analysis the R-square value of the model was R-squared 0.369134 and Adjusted R-squared 0.302597. Like wise another findings showed...
24 October 2017 3,721 14 View
Namaskar Kindly suggest methods of transformation for negative values. How to do reflect and log transformation of negative value.
24 October 2017 2,069 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View