Kindly provide scientific aspects of technique and protocol of yielding dental stem cells. Stalwarts are also requested to elucidate the techniques for harvesting and isolation of dental stem cells along with discussion of the sample selection criteria for dental stem cells. Guidelines for extraction of tooth for harvesting and post extraction protocols can also be mentioned in your answers.
Awaiting a healthy brainstorming discussion and response.
We have the same question at the moment. Unfortunately we couldn’t find a good receiving protocol for dental stem cell, but we have already got primary tissue culture from pulp cavity (using collagenase). We are going to get pure culture in the future. The magnetic sorting helps us with this problem.
What phase do you have till this moment?
HI Dear, I hope those articles may help
Article Evaluation of implant-materials as cell carriers for dental ...
Article TOOTH REGENERATION WITH DENTAL STEM CELL RESEARCH IN MINIATU...
I isolated mesenchymal stem cells (MSC) from dental pulp, apical papilla, periodontal ligaments and dental follicle. I used the explant method. I extracted the impacted third molars or premolars (in orthodontics purpose) and I put them in DMEM 15%.The teeth were disinfected with alcohol 70 degree (10 seconds) and were washed with PBS (three times).The explants were fragmented mechanical (with scissor) and after that were centrifuged (1100/min, 5 minutes). The MSC appeared in primary culture after 5-10 days.
Please read the article:
A.B. Boșca, O. Sorițău, A. Ilea, M. Moga, M. Cenariu, R.S. Câmpian, P. Virag, A. E. Pârvu. Effect of Curcumin and Epigallocatechin-3- gallate on stem cells derived from human periodontium. Annals of the Romanian Society for Cell Biology. Volume XIX, Issue 3, 2015, pp. 83 – 94
Dear Aman Chowdhry
pls check the link.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953626/
http://www.sciencedirect.com/science/article/pii/S1883195812000898
http://www.jaypeejournals.com/eJournals/ShowText.aspx?ID=4811&Type=FREE&TYP=TOP&IN=_eJournals/images/JPLOGO.gif&IID=371&isPDF=YES
Dear Aman Chowdhry
pls check the link.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953626/
http://www.sciencedirect.com/science/article/pii/S1883195812000898
http://www.jaypeejournals.com/eJournals/ShowText.aspx?ID=4811&Type=FREE&TYP=TOP&IN=_eJournals/images/JPLOGO.gif&IID=371&isPDF=YES
Dear Aman Chowdhry
pls check the link.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953626/
http://www.sciencedirect.com/science/article/pii/S1883195812000898
http://www.jaypeejournals.com/eJournals/ShowText.aspx?ID=4811&Type=FREE&TYP=TOP&IN=_eJournals/images/JPLOGO.gif&IID=371&isPDF=YES
Dear Dr Nirmala..
Ur link were extremly helpful.. i might be starting my work on yielding of stem cells very soon.. i will surely keep disturbing you with my queries.
Thanx for ur resources provided.
Best
Aman
Dear Dr Vsevolod Melekhin
Have been occupied with some unavoidable things... sorry for delayed response..i have started the process of procurement of consumables for the process. we have a plenty of scope in our university. just wanted inputs from scholars before we define our set protocols.
Dear Ilea Aranka
thanx for the article. I will ask u for more queries very soon.
Best
Dear Hala Hazzaa
thanx for the links/article... will surely use them in my manuscript as references.
Best