I have a query that whether there is any restriction enzyme (endo or exo) which can cleave both ssDNA or dsDNA?
... yes, there are a few enzymes that recognize and cleave single stranded DNA substrates: HaeIII is one example, a blunt end cutter with the recognition and cleavage sequence GGCC.
... yes, there are a few enzymes that recognize and cleave single stranded DNA substrates: HaeIII is one example, a blunt end cutter with the recognition and cleavage sequence GGCC.
Mr. Johannes Wöstemeyer, thanks for your answer.
Can you provide me a reference of any published literature indicating your statement.
I did use Hae III so many times and never thought it does. Cheers
Easy question, easy answer: K. Horiuchi, N.D. Zinder:
Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease. Proc. Nat. Acad. Sci. USA 72, 2555-2558 (1975).
Please be aware: Hemophilus instead of Haemophilus is part of the original title, a very frequent typo over the years. For your own texts I strongly recommend "Haemophilus", which is the correct genus name.
I can confirm Dr. Wostemeyer's answer. I just digested phix174 virion ssDNA yesterday with HaeIII and observed complete digestion of the circular ssDNA, resulting in several discrete bands that run between ~1-2 kb on a 1% agarose/TAE gel.
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