Are there any papers showing the use of TALENS with either GFP/RFP or any fluorescent protein for that matter?

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Why the digested solution of e-waste with aqua regia was diluted with 0.5M HNO3 before determine the metal contents using AAS or ICP-OES?

Several studies diluted the digested solution of e-waste but what is the purpose of this procedure?

31 January 2023 5,056 1 View

Is it ok to use expired formaldehye for fixation of voucher specimen and tissue samples for histological analysis?

I am taking gonad samples of fishes for histology to look at maturation stages. I couldn't find definite information if expired formalin can be used to make 10% buffered formalin or if it would...

15 October 2017 1,852 1 View

Any suggestion on fish sampling methods in fast flowing streams and nocturnal sampling

Streams are freshwater with fast flowing current (velocity 0.4 to up to 1 m/s), from main channels (C.20 metres wide, depth c. up to 5 metres in pools) to tributary streams (5-10m wide, mostly...

02 June 2017 1,207 3 View

How to rotate a transversal coupling matrix of a filter to find out a folded coupling matrix using matrix rotation?

I am trying to use Givens Rotation and trying to zero a specific element in the matrix. But I can not do it correctly. Can anyone please tell me the steps with any example? Reference is to the...

17 April 2017 8,621 2 View

Otolith microchemistry - application in freshwater fish ecology

Our objective: To determine whether the freshwater fish I am studying uses spawning habitat that is different from the feeding habitat. The plan is to use otolith microchemistry although the...

01 January 1970 1,293 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View