Are there any papers of Guekensia granosissima (Southern ribbed mussel) or Geukensia damissa (Atlantic ribbed mussel) in the mangrove system?

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Hello everyone! Please, which professional software can I use to do an online survey for data collection?

Hello everyone! Please, which professional software can I use to do an online survey for data collection? I would appreciate a survey that is easy to use and can be used by participants from...

10 June 2024 6,196 6 View

What is this peak at 2430 in my Raman spectra (Oxygen evolution reaction electrode in-situ electrolysis)?

I can't find any definitive peak explanation for the one I see at 2430 1/cm in the attached file. It is common amongst my ionomer, which is a Versogen PAP polymer based on a quaternary ammonium...

10 January 2024 7,222 1 View

Why do some researchers not use RIPA Buffer to lyse exosomes for BCA or WBs?

Hello, I've been trying to isolate exosomes from cell culture supernatant. I have spoken to other researchers who do exosome work and found that they don't use RIPA buffer to lyse the eVs when...

01 November 2023 1,569 2 View

Concentration of Sodium hyaluronate to make hydrogel?

Now I have sodium hyaluronate(SH) powder which is 7kda to 12kda and 75kda to 90kda. What concentration will be suitable to make SH solution to make hydrogel with good mechanical performance?

26 September 2023 7,696 0 View

RPM to form Sodium hyaluronate hydrogel?

Now I have Sodium hyaluronate solution from 0.1%to 1%. What range of RPM would be feasible to make sodium hyaluronate hydrogel by using a high speed centrifuge?

26 September 2023 6,172 0 View

How to get a sodium hyaluronate solution?

Now I have sodium hyaluronate powder which is 75kda to kda, what solvent should I choose to make a 1% sodium hyaluronate solution. Is PBS solution or water suitable in this case?

24 September 2023 2,754 1 View

What is the best approach to analyze the relative fold change of gene expression when time is the only treatment?

I have an ongoing gene expression study and have had difficulty finding an appropriate way to analyze the data. For context: I want to observe the relative fold change in expression over time for...

11 June 2023 8,848 3 View

Literature review on insect fauna of mango ?

introduction

23 May 2023 8,837 0 View

Can anyone provide recommendations for poly A tailing RNA?

I am looking to use poly A polymerase (NEB:M0276S) to add a poly A tail to RNA of plant viruses extracted from total RNA of plants. The purpose here is to perform 3' RACE downstream. Obviously, I...

22 June 2022 1,200 0 View

Ansi 208-2016?

ASTM 1037

05 June 2021 3,931 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities?

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities? How can ecological and recreational functions be introduced into the landscape and...

23 July 2024 7,292 4 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View