What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

20 November 2019 7,210 3 View

Can any one please suggest me that for micropore analysis of activated carbon which model gives relatively accurate value ?

I am new to micro-pore analysis. Recently, I have carried out low pressure isotherms (N2 @ 77 K and Ar @ 87 K) on activated carbon using a Quantachrome instrument. However, when I chose the...

21 April 2019 2,699 4 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Does anyone work with Diniobium Carbide (Nb2C)?

I would like to know if material is electrical conductive on nanosize domains. How different it is from NbC is Nb2C? Any literature?

19 June 2018 3,091 2 View

In your opinion, should ALL research articles be shared and accessed freely? Please give a reason for your answer (why)?

This will throw more light on the open access policy of publishing institutions

12 June 2018 7,439 3 View

Is it possible to create a part from an orphan mesh?

14 June 2016 1,343 3 View

Attached is an image of PPF stained with PTA. The PPF has nano cellulose particles. My question is this artifact from the stain?

Stained with PTA for 15 minutes. Bulk material is PPF with nano cellulose crystals.

22 May 2016 1,559 1 View

In the attached paper, the clue is Wernicke that guarantees thiamine deficiency. Was this sought as etiology?

Wernicke has occurred in hyperemesis with and without hyperalimentation and thiamine deficiency (TD) is the etiology of the hyperemesis. Since emotions are generated in the limbic system that is...

21 May 2015 6,476 1 View

No title

01 September 2014 7,367 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View