08 August 2017 10 9K Report

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What is the maximum overall column length suitable for LC-MS using a 50 mm HILIC column?

Curious about your research experiences. Ideally this is for ESI-QTOF electrospray ionisation. This will differ based on type of columns and equilibration as per dimensions, flow rate and size of...

09 October 2017 5,600 6 View

I want to test my HILIC column with different mobile phase %. Do i need to equilibrate 2 hrs per mp %?

the water layer would have formed using 85%. Is the same amount of water layer formed by each acetonitrile %.what I am asking is for 97% if I equilibrat for 2 hrs then can I equilibrate for 30 to...

08 September 2017 7,441 11 View

How much of a water layer is required in HILIC column to effectively bind hydrophobic compounds?

,Tried 3% water layer but equilibration time is time consuming. Analyte had a curved out looking shape such as an upside boat that continued for 2minutes to reach the baseline. steep then smooth...

08 September 2017 4,792 9 View

Are there any literature sources on coupling of HILIC column to another separate stationary phase?

Anal Chim Acta. 2017 May 15;967:12-32. doi: 10.1016/j.aca.2017.01.060. Epub 2017 Feb 7. Recent advances in stationary phases and understanding of retention in hydrophilic interaction...

08 September 2017 8,806 6 View

What research techniques have you learned over the years allowing you to quickly finish research?

For me cite them right website for referencing got lab reports done easy with references. Learned web of science makes results so narrow and easy to find. Google scholar let me find cited articles...

08 September 2017 6,102 2 View

For my HPLC UV detection can I apply 0.1% formic acid and 10mM Ammonium acetate at pH 4 to see what effect the buffer combo can have in HILIC?

I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the...

08 September 2017 834 4 View

What are some good examples of articles for moderately polar compounds separated by HILIC?

LogP

08 September 2017 869 5 View

What are the typical To void volume markers used for mixed mode chromatographic columns that do not retain hydrophobically/ hydrophilically?

This situation can be applied to 2D chromatography. If there is a T-piece can two To void volume markers be used to measure that volume for sample to reach the detector. Some research applies...

08 September 2017 10,129 5 View

I have an impurity from a purchased bacterial standard. What methods do you use to analyse if it is a standard besides HPLC-UV detection?

Which methods are the fastest and easiest to do to save time. The LC-MS is broken for now and awaiting repair otherwise I would fragment the impurity with different m/z ionisation.  In the...

08 September 2017 7,261 2 View

Are there any infra red detectors for HPLC or UV+IR detector for liquid or gas or supercritical fluid chromatography?

Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...

08 September 2017 7,592 5 View

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