Are there any non-metabolizable analogues of arabinose that i can use to induce my GOI under the control of pBAD promoter in E.coli?

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Frederik De Bruyn

Does it matter for your experiment? I would suggest using another expression system than testing various analogues.

Vijay Jayaraman

I am growing it on selective carbon source. So arabinose addition wouldnt fall in the right experimental design. Yes, changing the expression system would be best option. Was just trying to find if there are any analogues like for eg. IPTG for lac operon. Thanks for your interest.

Barbara Di Ventura

use the light-inducible version of AraC, called BLADE:

https://www.nature.com/articles/s41589-021-00787-6