Vijay Jayaraman

8 Questions 13 Answers 0 Followers

Questions related from Vijay Jayaraman

Structure of protein complexes: using phase from one subunit to solve the other. Details?

When the structure of one of the subunit in a heterodimeric protein complex is known, can that phase be used to solve the structure of the other subunit for which no homology exists? In that case...

30 November 2015 439 2 View

Is there a way to find out the optimal occupancy of the molecule when trying to solve a structure?

I am trying to solve the structure of a protein. Resolution is 2.3A. The average B factor is around 56.0. Wilson B estimate is 36.4. I have a good Rwork of 0.2 and Rfree of 0.24. I have modeled...

03 August 2015 7,368 7 View

Can shRNA be cloned in any stable plasmid and made use of for gene silencing?

I understand that shRNAs can be cloned in either specific commercially available vectors or even introduced through lentiviral mediated transduction process. However, if I have to opt the first...

30 July 2015 1,857 2 View

Are there any non-metabolizable analogues of arabinose that i can use to induce my GOI under the control of pBAD promoter in E.coli?

Fucose is a non-metabolizable sugar but they repress the pBAD promoter rather than inducing it.

17 June 2015 8,725 3 View

How is gyromagnetic ratio for nucleus measured experimentally?

And how to visualize this parameter?

03 June 2015 3,108 4 View

What decides the kind of regulation for a particular biological function/phenotype?

Regulation of a biological function can be at transcriptional, translational or at the protein level (PTM or allostery). What are the constraints imposed by the phenotype that selects the nature...

02 June 2015 6,401 4 View

Can we use Yeast nitrogen base without amino acids as a minimal media for E.coli?

Is there anything in the media that can act as carbon source for E.coli. myo-inositol is one but its concentration is very less.

28 May 2015 3,336 6 View

Why has the life cycle of Plasmodium berghei evolved to be 24hrs rather than 48hrs for the human pathogen P. falciparum?

What are the evolutionary constraints that determine the life cycle of a pathogen like plasmodium?

25 May 2015 8,744 4 View