Are there any methods to isolate gene related gastritis?

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Analytical and experimental results?

When comparing analytical and experimental results, are there any guides to decide the agreement between both results? I mean what the ratio of analytical/experimental results should be to say...

04 May 2017 4,972 4 View

Could anyone provide proposed values of sand Young modulus?

Could anyone provide proposed values of sand Young modulus with respect to depth and relative density? For example, Poulos suggested Es = (6–8)z (in MPa) for very dense sand. Any other references...

11 December 2016 6,157 0 View

Interface elements with reduced strength?

Can interface elements be added between two layers of the same soil having the same properties. The top layer is moving while the bottom one is stable just like the direct shear test.

10 November 2016 9,868 6 View

For laterally loaded piled raft, is there any relationship between soil reaction and the magnitude of axial force?

For laterally loaded piled raft, is there any relationship between soil reaction and the magnitude of axial force. Or, does axial force affect soil pressure measured along the pile length?

31 December 2015 1,191 4 View

How do I find the Sand Young's modulus?

I'd like to use Direct shear test to find sand Young's modulus in the lab. What is the correct procedure and steps to do that?

07 August 2015 1,305 5 View

Does anyone have an idea on how to calculate the force exerted on a fixed-tip free-head pile caused by a moving mass of water?

02 March 2015 9,342 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

Ihsan E Al-Saimary

There are many of molecular techniques