Are there any instances wherein both Chlamydia trachomatis and Treponema pallidum coexist in causing non-gonococcal urethritis?

19 October 2020 0 8K Report

What is/are the specific bacterial detection method that can be used in the detection of both species?

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What is the current method for identification of Genital Chlamydia serovars?

I am working on Chlamydia trachomatis characterization and I would like to use molecular methods rather than serologic method. Can anyone provide me with the protocol for identification of...

17 July 2020 2,953 1 View

How can chlamydia control the host cell staying in the phagosome?

Recently become quite interested in the lifecyle and molecular bio of chlamydia. Is it right that the chlamydia gets into host cell through endocytosis and live within phagosome? Have read paper...

04 May 2019 7,736 4 View

Is it possible to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells?

I have been trying to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells using the MOMP and Plasmid primers respectively without success. So far only...

01 February 2018 4,601 2 View

Find where this DNA fragment comes from, sequence from 1998?

This DNA fragment (document attached) comes from which organism? What is the complete strain name that it comes from?. If several organisms with identical sequence are found choose the one where...

10 November 2017 9,079 3 View

Does chlamydia infection trigger any upregulation in glycolysis gene expression in the host cell?

I know chlamydia uses some glycolysis enzymes ,that they lack, from the host cell, does that uptake of enzymes trigger any homeostatic event from which the host cell would upregulate some genes...

09 October 2017 5,276 1 View

Why does the slope looks like in vii7 (realtime)?

The test is for detecting the direct qualitative for Chlamydia trachomatis and Neisseria gonorrhoea. We use FAM (CT), JOE (NG) reporter and CY5 (beta for control). Results are reportable after we...

07 June 2017 9,398 2 View

In an indirect diagnosis for syphilis, why should we first perform a nontreponemal test followed by a treponemal one?

The non Treponema based assay is highly sensitive, but not very specific so that if we have a positive result we must perform an additional test. Why not to make the Treponema based one immediately?

11 December 2016 3,816 1 View

How long would it take to lyse HeLa cells with DI water?

I am currently working with Chlamydia infected HeLa cells. The protocol given to me by my PI states this should be happening within 2-10 min. However, he and I have given it a trial run and even...

06 July 2016 4,005 2 View

Is there an reference article with primers for C. trachomatis?

Primers for C. trachomatis

03 April 2015 1,726 3 View

How can I increase the performance of my Eubacterias PCR on Patients sample?

I explain, i'm doing PCR on Chlamydia trachomatis. The PCR on strains works fine, i got large bands on gel, and when i'm sequencing it, the result is perfect.Then i had to do the PCR on patients...

23 February 2015 2,691 4 View