Are there any instances wherein both Chlamydia trachomatis and Treponema pallidum coexist in causing non-gonococcal urethritis?

19 October 2020 0 8K Report

What is/are the specific bacterial detection method that can be used in the detection of both species?

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Which sample is a choice for the diagnosis of PID due to Chlamydia infection?

Chlamydia trachomatis in women is symptomatic and mainly causes cervix erosion. The microorganisms are obligately intracellular and can not grow on ordinary or routine culture media. As well, it...

28 February 2023 9,869 3 View

Why does diagnosis of NGU caused by Chlamydia trachomatis is more commonly associated with men?

Chlamydia trachomatis infections include nongonococcal urethritis (NGU) that is frequently seen in adult men. Cases and rates are higher in men than women, but what is the clinical evidence and...

18 October 2020 5,545 0 View

What is the best primer sequence for detecting chlamydia trachomatis infection using PCR technique?

Detection of DNA samples from vaginal swabs of pregnant women

01 September 2020 9,415 3 View

What is the current method for identification of Genital Chlamydia serovars?

I am working on Chlamydia trachomatis characterization and I would like to use molecular methods rather than serologic method. Can anyone provide me with the protocol for identification of...

17 July 2020 3,136 1 View

Why the bacterium Treponema pallidum is difficult to culture?

Treponema pallidum subspecies pallidum (T. pallidum), the causative agent of syphilis, is one of the few major bacterial pathogens that has not been cultured.

03 May 2020 7,910 69 View

Problems with Conjugated Proteins and Unconjugated, please Help?

Problems with Conjugated Proteins and Unconjugated I engineered an E.coli 47kDa Treponema pallidum protein that works perfectly on ELISA solid-phase detecting positive sera w/o background on...

02 March 2020 2,730 5 View

Any body knows where I can get genomic DNA for Treponema pallidum and Haemophilus ducreyi?

Need genomic DNA from isolates of these organisms for molecular assay development

02 October 2019 4,814 1 View

What RPR/TPHA titre is an indication for CSF analysis in asymptomatic patients with syphilis?

Treponema pallidum apparently invades the CNS in 40% of patients with syphilis. With time there have been changing guidelines as to what is the serum RPR or TPHA titres that indicate the need for...

16 August 2019 3,495 1 View

How can chlamydia control the host cell staying in the phagosome?

Recently become quite interested in the lifecyle and molecular bio of chlamydia. Is it right that the chlamydia gets into host cell through endocytosis and live within phagosome? Have read paper...

04 May 2019 7,899 4 View

Is it possible to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells?

I have been trying to detect Chlamydia trachomatis from genomic DNA extracted from exfoliated cervical cells using the MOMP and Plasmid primers respectively without success. So far only...

01 February 2018 4,675 2 View