Treponema pallidum subspecies pallidum (T. pallidum), the causative agent of syphilis, is one of the few major bacterial pathogens that has not been cultured.
Unlike most bacteria, the organism that causes syphilis, Treponema pallidum, cannot be cultured in the laboratory. Due to this limitation, research on T. pallidum and syphilis has not benefited from new molecular approaches that have become available in recent years.
Hi Dr. T. Pallidum is very fragile bacteria and it has a long generation time. So the cultivation of this bacteria is not recommended in medical settings
The genus Treponema consists of obligate anaerobes, but those species that are pathogenic to humans may be microaerophiles. Treponema are heterotrophic bacteria that mainly metabolize through fermentation. They can use a variety of carbohydrates and amino acid as their carbon source and energy. Treponema species are difficult to grow in artificial culture media, the growth of some species requires long chain fatty acids from serum, and other species require branched-chain fatty acids. Treponema denticola, Treponema vincentii, and Treponema scaliodontum require cocarboxylase in serum
I'm consistent with my colleagues as it lacks essential genes that code for enzymes involved in tricarboxyluc acid cycle and oxidative phosphorelation.
Because it lacks the genes needed for TCA cycle and oxidative phosphorylation. D. Because it requires extremely low temperature at which water freezes.Jun 9, 2017
Because it lacks the genes needed for TCA cycle and oxidative phosphorylation. D. Because it requires extremely low temperature at which water freezes.Jun 9, 2017.
You can see the following link for more information
It is a big problem that microbogies face because these bacteria lack the genes which encode metabolic activity, and thus lead to impairment in their ability to grow in the usual culture media.