How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to inter pain intensity data using NRS when patients report their pain in spectrum?

I have been working on a pain intensity study and I wanted my patients to report their pain intensity using numerical rating scale. many patients have reported thier pain in spectrum like 2 to 3...

13 February 2021 6,598 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

I'm looking for a dataset of eeg recorded during the pain stimulation. Anyone can help me to find it?

I'm looking for a dataset of eeg recorded during the pain stimulation (prefered the cold pressure pain stimulation). Anyone can help me to find it?

25 January 2021 301 2 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Rerfering to somatic symtom disorder: When is a patient preoccupied or simply adequetely worried? How do you define preoccupation in this context?

In order to fulfill criteria for Somatic symtom disorder (DSM 5) a patient needs to be preoccupied with symtoms or their health. Reasonably a symtom like pain does make you preoccupied to some...

19 November 2020 4,195 4 View

What are the 2020 most recommended systematic review and meta-analysis articles on PAIN?

We are starting an investigation, in the field of physiotherapy, on the evaluation and clinic of pain. We are interested in knowing which diagnostic evaluation scales are being evaluated. We...

18 November 2020 2,258 1 View

Multiple regression - What to do with 2 similar independent variables?

17 November 2020 1,938 2 View