I know of linear solvation equations but not in detail. I know for reverse phase and hilic there is hydrophobic subtraction model.
If so would coupling reverse phase column to hilic column increase or decrease hydrophilic and hydrophobic interactions compared to a single mixed mode column. What about cost effectiveness of hyphenated columns vs mixed mode columns in purchasing operation costs and can isocratic methods work well on mixed mode columns compared to gradient elution?.
Perhaps??? It is the molecules to be separated that determine the mode of separation. Thus, you could have a sample matrix that contains molecules that requires HILIC as well molecules that require reverse phase separation. It depends on this mixture.
Wouldn't people just perform 2 separate HPLC runs? 1 HILIC and the other reverse phase.
Usually it depends on the equipment of hplc available. There was a Yang 2008 paper on 2 columns coupled to 2 detectors with separate injections with 2 detectors per column.
Why a decrease in peak capacity when separation time is extended and k' capacity factor related to resolution increase?. If there is such material I would like to read more.
Thank you for the information source. I feel it is quite equation heavy to understand. I found that before but I overlooked what was inside the contents.
I think the peak capacity decreases because the second column does not get the same amount of analyte hence the k' of the second columns is lower than the k' capacity factor of the first. Hence the alpha is smaller than at >1.5 to 2.0 and k' ideally 2-10. Am I correct in interpreting this concept?
Below is an extra article on Nc1 and 2 equation of product of one D and two D for peak capacity equation (permutations).
Li, Xiaoping, Dwight R. Stoll, and Peter W. Carr. “A Simple and Accurate Equation for Peak Capacity Estimation in Two Dimensional Liquid Chromatography.” Analytical chemistry 81.2 (2009): 845–850. PMC. Web. 16 Aug. 2017.
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