I want to estimate Arginine in plant tissue using any equation r formula based on nitrogen content in plant.
thanks
Are you interested in free amino acids, or arginine which has been incorporated into proteins? Or both?
I'd suggest measuring it rather than estimating - free amino acid concentrations vary quite a bit depending on plant species, and the type of N the plant has been taking up. My guess is that any estimate based on total N content alone would be extremely inaccurate.
Free amino acids can be extracted using 20% methanol (e.g. Soper et al 2011 http://dx.doi.org/10.1071/FP11077).
If you're interested in arginine which has been incorporated into proteins, then the best approach is to do a Kjeldah acid digestion, followed by measurement.
There are quite a few approaches to then quantify the amino acids using liquid chromatography or gas chromatography. If you don't have access to that, then there are colorimetry methods specific for arginine (eg Mira de Orduña 2001 J Agric Food Chem. 2001 Feb;49(2):549-52.).
Dr. Ali.
Arginine is estimated on the basis of the ratio of nitrogen in protein 16%
The ratio of arginine = nitrogen× 3.2
(Gerhardt,2010)
I didn,t find this paper when i search for , furthermore there are many papers mentioned that the use of the formula:
total protein= 6.25* N value calculated by kjeldahl method gave incorrect value for total protein because The use of a single factor, 6.25 is confounded by two considerations; First, not all nitrogen in foods is found in proteins, nitrogen is also contained in other compounds, such as amides, free amino acids, peptides, nucleic acids, nitrogenous lipids, ammonium salts, nucleotides, nitrates, creatine, choline and secondary compounds.
thanks
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