09 February 2024 1 1K Report

Any links or table will be appreciable

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How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

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How to determine the quantity/amount of secondary phase based only on peak intensity?

I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...

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Why is my TE-eluted cloned plasmid (isolated via miniprep) not freezing at -20 degree celsius? Is it normal?

I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...

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How can machine learning be employed in the field of natural sciences?

Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...

18 June 2024 1,897 4 View

Can someone help me with the alpha amylase inhibition assay?

I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...

27 May 2024 9,348 0 View

How can I find permission and access to parenting style questionnaire?

I need to use and adapt the parenting style questionnaire in my ongoing research project, can somebody please help me regarding gaining permission and access to them as I am unable to find the...

25 May 2024 6,304 1 View

How can i find co author?

I need help for writing a chapter.

15 May 2024 3,963 2 View

Cell Culture Debris? or Contamination?

I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...

12 May 2024 9,279 2 View

How to improve RNA Integrity in bacteria using Purelink RNA mini kit?

I am using Purelink RNA mini kit (Fischer Scientific) to extract total RNA from bacteria. i need to get better RNA integrity number like more than 7, but for only one sample I got 7.4, for other...

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Can PVA be used as an additive in Aluminum Powder for achieving higher Sinter density?

I am an undergrad student and I need to suggest optimum amount of PVA to be added in Aluminum powder for achieving improved sinter density. Your guidance will mean a lot to me. Thanking in...

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