Are there any disadvantages pETDuet-1 vector to do coexpression, especially for purification step.
I'm thinking to add phospates, corrosion inhibitors, organic acids, some inorganic compounds and glycol in to my agent. But without surfactants I've got concerns about the extingusihing capacity....
24 May 2023 9,448 3 View
We are researching on a water based fire extinguisher. The system contains water mist noozles.
19 October 2020 3,774 1 View
I would like to use RADII=BONDI with IEFPCM solvation for trans-4-Methylcyclohexanol. I am using for optimization B3LYP/6-31G(d) or B3LYP/6-311+G(2d,p) methods (I tried both of them). But I get...
13 February 2018 5,674 0 View
Hi everyone, We are dealing with a 2D analysis. We applied the force vertically onto an edge. The threaded sides are in frictional contact with a screw. Although we can't find any geometric...
29 November 2016 2,531 14 View
In literature most of the researchs about treatment response using DSM-IV or DSM5 criterias, CGI-I, PTSD-I scores but none of seperate treatment response negative or positive as binary. In...
05 September 2016 1,158 16 View
There are some case reports about anorexia nervosa with gastrointestinal or brain tumor. Is there any relationship?
28 June 2013 8,945 1 View
The Sumerian term 𒌦 (kalam), which means ''homeland" or "mainland," has possible linguistic ties to Proto-Altaic and Proto-Turkic concepts that evoke notions of proximity, family, and permanence....
01 January 1970 4,691 1 View
We are working on a piping sprinkle fire supression system for an %100 electrical bus.Currently we are using C02(g) for physical suppression with 50 bar but we can not obtain the measurment in...
01 January 1970 9,302 9 View
I want to test credibility of this important phenomenon: "Women's handcrafts played a pivotal role in the development of cuneiform signs, serving as the content of the writing system. This is...
01 January 1970 6,862 0 View
There are three universal energies: 1. **Proto Electricity**: Associated with Uranus, this energy is characterized by the planet's unique axial tilt and its elliptical orbit, suggesting a profound...
01 January 1970 5,698 0 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Purification of gst tagged protein
25 July 2024 1,720 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
22 July 2024 5,334 6 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View