How will I calculate the energy balance of a parabolic trough collector?

Not all the solar energy that fall on the parabolic trough collector is been utilized. Some are being scattered while others are absorbed.

09 September 2019 6,520 9 View

I want to construct a solar adsorption refrigerator using parabolic trough. How can I assemble my adsorption bed in the collector?

To enable me have high heat generated in the adsorption bed I decided to use parabolic trough solar collector for my adsorption solar refrigerator.

12 March 2019 4,614 4 View

Anyone knows who makes these piles of small stones?

I am attaching a couple of photos of small stone piles - the piles have a diameter of up to 10 cm, and can be up to 4 cm high (but sometimes they are quite flat). The small stones are about 2-5 mm...

16 May 2017 4,167 15 View

Does anyone know the effect of one broken electrode in a pulsed field gel rig?

14 August 2015 4,841 5 View

Harnessing the available solar energy in my place for domestic use.

I am in a region where there is high solar radiation and I wish I could design and construct something that will harvest and use the energy for domestic use and will also serve as my thesis work.

01 January 1970 4,478 9 View

Solar adsorption refrigeration.

Research has shown that there is wide scope for further development to improve the thermal performance of adsorption cooling systems including development of new adsorbent materials,...

01 January 1970 9,956 18 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View