Is there any selective media to grow filamentous fungus only (not with yeast)?

22 November 2020 5,370 7 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

I want to simulate a VAR model. How do I choose the coefficients to ensure stability?

I know how to compute eigenvalues to test stability. The question If i have for example a 10 node model with 5 lags, how do i choose stable parameters without just guessing.

06 October 2020 5,238 6 View

What is the pebbling number of a cycle graph with 4 vertices?

i need the number of pebbles for a cycle graph with 4 vertices

24 September 2019 4,188 1 View

Anyone know where the micro tensile testing machine available?

In Chennai where micro tensile testing machine available

19 August 2019 3,926 2 View

How do I etch nichrome thin film on polyimide(kapton) substrate? Can someone explain the procedure?

I'm currently working on fabricating etched heating element for a project work.

08 August 2018 1,746 3 View

Is there an association between non-vegetarianism and violence?

Is there any scientific study of this possible association? There have been informal discussions and surmises, but a serious study with appropriate controls to cancel out extraneous factors is...

29 May 2018 903 3 View

What could be the possible reasons for getting 20 unit less value of mass of pure compound which is well charecterised by NMR?

My compound is well charecterised by NMR but in case of ESI-mass spectrum it is coming 20 unit less then the expected mass. Molecule is not having any associatio with water. I could not find any...

12 September 2017 6,260 2 View

Different significance values on SPSS and PLS SEM. Please help.

On SPSS I am getting a result that shows no significant impact of the independent variable on the dependent. But when I am running the data on SmartPLS I am getting a significant impact. Please...

24 July 2017 5,505 3 View

Is gender a dichotomous variable? If yes, Can t-test be run on single large sample dichotomous variable (demographic)?

My colleague's thesis consists of a situation where she has performed a t-test to analyse the differences between male and female respondents' responses with respect to all the independent and...

19 July 2017 2,255 6 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View