Amorphous XRD Data?

Dear colleagues, I analyzed my material using XRD analysis and the material has shown amorphous behavior , how can I specify the composition of the material is there any extra analysis I can...

24 December 2020 1,280 10 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View

Can you put cells from -80 to liquid nitrogen?

Hi all, I have kept cells in -80 for several months, can I transfer them to liquid nitrogen now after all this time? I am afraid they would not survive.. I appreciate your input Thanks!

12 July 2020 3,612 2 View

Solar cell exciton binding energy ?

30 April 2020 1,620 12 View

Can I make a primerset (for RT-qPCR) for one single transcript variant? If yes, how?

I am trying to perform a RT-qPCR and I would like to design a primerset for one single transcript variant for RT-qPCR. The problem is that it is very difficult to get primers that only bind on one...

22 April 2020 3,071 4 View

Is there a comprehensive list of qualitative research methodologies to be found?

I am searching for a (relatively) comprehensive taxonomy or list of qualitative research methodologies. A list that goes beyond Creswell's "five approaches" (likely not exhaustive, but nonetheless...

24 November 2019 9,989 8 View

How would you suggest that an author be transparent about ideas that came up only during the review process, and by a reviewer rather than the author?

I am conducting a qualitative study. The manuscript was returned to me from the initial review with the recommendation that I revise and resubmit. So far so good. One of the reviewers, however,...

23 September 2019 7,963 8 View

Too many bands after digestion with restriction enzymes?

Hello Everybody, I am in the process of extracting a natural plasmid containing the CTX-M 1 gene. I do not know what plasmid (type/Inc etc) is present in my sample but I have been successful in...

28 May 2019 832 3 View

How can you prevent cells from clumping on a coverslip of a 24 well plate?

Hi, I'm trying to stain my cells after transient transfecting them but each time I get the cells either over confluent, or clumped in the middle even though I swirl the dish in an 8 shape to...

15 March 2019 6,585 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Rearranging abbreviation list in LaTeX ?

Hi, I'm trying to rearrange an abbreviation list for a manuscript such that it would be listed in alphabetical order. Is there a function that does this automatically or is manual my only option?...

18 February 2021 4,870 2 View

Is there any free software for behaviour analysis of zebrafish ?

I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?

16 February 2021 1,541 1 View

Is there any kind of data available for connection properties like signal strength between UE and RAN?

Is there any kind of data available for connection strength between UE and RAN? I am looking for signal strength, the geographical coverage of RAN's in a given Tracking Area, Coverage Area over a...

16 February 2021 1,073 5 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View