I am looking to perform RNA extraction on whole blood samples currently in Paxgene tubes using a Qiagen Blood RNA Extraction kit designed for use with Paxgene tubes. The protocol calls for 2 steps that require a shaker-incubator at 55-65C and 400-1400 rpm for only 5-10 minutes. We have asked other neighboring labs to use theirs but it is difficult to find a time that works for everyone, and shaker-incubators are very expensive. We do have an incubator, it just doesn't shake, so I'm wondering if we can just leave them to incubate at the required temp and then every minute or so take them out and manually shake them for a bit then put them back in.
You definitely can shake your samples manually. should not be a problem.
Jaroslaw Krol Thanks! Much appreciated. Have you used this kit before/done manual shaking instead of shaker-incubator?
Hi Julien,
First of all I didn't use this kit before. We use K2Edta tubes for isolation and do RNA isolation with trizol in our lab. And we do manual shaking on some steps, just like yours and we had no problems.
I think you can shake your samples manually. I don't think you will have problem.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction