I am interested in studying organisms dependent on alkaline (high) pH values > pH 9 that can be grown in the lab and ideally have available DNA sequence information and be thermophiles.
Hi, for a fast doubling time I would look at Clostridium paradoxum if its an anaerobe you are after. The problem with most of these organisms are they are not genetically tractable to date.
You can grow such organisms in lab with a short doubling time. However, it all depends on what your looking for (which is not clear from your question)
We have published several anaerobic alkaliphilic thermophiles with doubling times between 10 and 20 min.
Li, Y., L. Mandelco, and J. Wiegel. 1993. Isolation and characterization of a moderately thermophilic anaerobic alkaliphile, Clostridium paradoxum, sp. nov. Int. J. Syst. Bacteriol. 43:450-460.
Li, Y., M. Engle, L. Mandelco, and J. Wiegel. 1994. Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile. Int. J. Syst. Bacteriol. 44:111-118.
Engle, M., Y. Li, C. Woese, and J. Wiegel. 1995. Isolation and characterization of a novel alkalitolerant thermophile, Anaerobranca horikoshii gen. nov. sp. nov. Int. J. Syst. Bacteriol. 45:454-461
Engle, M., Y. Li, F. Rainey, S. DeBlois, V. Mai, A. Reichert, F. Mayer, P. Messmer, and J. Wiegel. 1996. Thermobrachium celere, gen. nov., sp. nov., a fast growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe. Int. J. Syst. Bacteriol. 46:1025-1033
Svetlitshnyi, V., F. Rainey, and J. Wiegel. 1996. Thermosyntropha lipolytica gen. nov. , sp. nov., a lipolytic, anaerobic, organoheterotrophic, alkalitolerant thermophile utilizing short- and long chain fatty acids in syntrophic coculture with a alkaliphilic and thermophilic bacterium. Int. J. Syst. Bacteriol. 46:1131-1137.
Cook, G. M., J. B. Russel, A. Reichert, and J. Wiegel. 1996. The intracellular pH of Clostridium paradoxum, an anaerobic alkaliphilic, and thermophilic bacterium. Appl. Environ. Microbiol. 62:4576-4579
Wiegel, J. 1998. Anaerobic alkali-thermophiles, a novel group of extremophiles. Extremophiles 2: 257-267
Mesbah, N. and J. Wiegel. 2006. Isolation, cultivation and characterization of alkalithermophiles. IN: Methods in Microbiology. (Ed. An Oren and F. A. Rainey) Academic Press / Elsevier. pp. 451-468
Mesbah N. M. , S. H. Abou-El-Ela, and J. Wiegel. 2007. Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Microbial Ecology 54: 598-617
Mesbah N., M. David, B. Hedrick, A. D. Peacock, M. Rohde, & J. Wiegel,. 2007. Natranaerobius thermophilus gen. nov. sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt. and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov. Int. J. Syst. Evol. Microbiol. 57: 2507-2512
Mesbah, N.& J. Wiegel. 2009. Natronovirga wadinatrunensis gen. nov. sp. nov. and Natranaerobius trueperi sp. nov., two halophilic, alkalithermophilic microorganisms from soda lakes of the Wadi An Natrun, Egypt. Int. J. Syst. Evol. Microbiol 59: 2043 – 2049
Mesbah, N., G. Cook and J. Wiegel. 2009. The halophilic alkalithermophile Natranaerobius thermophilus adapts to multiple environmental extremes using a large repertoire of Na+ (K+)/H+ antiporters. Molecular Microbiology 74: 270-281(with mini commentary by T.Krulwich)
Bowers, K.J., N. Mesbah, and J. Wiegel. 2009. Biodiversity of poly-extremophilic Bacteria: Does combining the extremes of high salt, alkaline pH and elevated temperature approach a physico-chemical boundary for life? Saline Systems 5:9 (OPEN RECORD)
Wiegel, J. 2011. Anaerobic alkaliphiles and alkaliphilic polyextremophiles. Chapter 2,4 IN: Handbook of extremophiles. (Ed.-In-Chief K. Horikoshi) Springer Verlag Tokyo. pp 81-98
Mesbah, N. M. and J. Wiegel. 2011. Halophiles exposed concomitantly to multiple stressors: Adaptive mechanisms of halophilic alkalithermophiles. Chapter 14 IN: “Halophiles and Hypersaline Environments: Current Research and Future Trends”, (Eds A. Ventosa, A. Oren and Y. Ma) pp. 249-274
Bowers, K. amd J.Wiegel. 2011. Temperature and pH optima of extremely halophilic Archaea: A mini-review. .Extremophiles 15:119–128 DOI 10.1007/s00792-010-0347-y
Mesbah, N.M. and J. Wiegel. 2011. Biochemical Characterization of the Na+-translocating F1FO-ATPase from the Anaerobic, Halophilic, Alkalithermophile Natranaerobius thermophiles. Biochim Biophys Acta 1807:1133-42
Zhao, B., N. M. Mesbah, E,Dalin, L. Goodwin, M. Nolan, S. Pitluck, O. Chertkov, T. S. Brettin, J. Han, F.W. Larimer, M. L. Land, L.Hauser, N. Kyrpides, and J.Wiegel. 2011. Complete Genome Sequence of the Anaerobic, Halophilic Alkalithermophile Natranaerobius thermophilus JW/NM- WN-LF. J. Bacteriol. 193: 4023-4024. http://jb.asm.org/cgi/content/abstract/193/15/4023
Mesbah, N.M. and J. Wiegel. 2012 Life Under Multiple Extreme Conditions: Diversity and Physiology of the Extreme Halophilic Alkalithermophiles. Appl.Environ Microbiol 78: 4074-4082
Pugin, B., J.M . Blamey, B.K. Baxter, and J. Wiegel. 2012. Amphibacillus cookii sp. nov., a facultatively aerobic, sporeforming, moderate halophilic, alkalithermotolerant bacterium from Great Salt Lake, Utah. Int. J. Syst. Evol. Microbiol. 62:2090–2096