I'm trying to find out if GPCRs are involved in the bioluminescence pathway of Dinoflagellates. Since the cells need to be alive, I need to target the GPCRs in vivo.
On the agonist side you could try for example forskolin, which would mimic Gs activation.
http://www.ncbi.nlm.nih.gov/pubmed/12825829
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Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
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