Mariska Beekman

3 Questions 4 Answers 0 Followers

Questions related from Mariska Beekman

Are there agonists or antagonists that work for all GPCRs?

I'm trying to find out if GPCRs are involved in the bioluminescence pathway of Dinoflagellates. Since the cells need to be alive, I need to target the GPCRs in vivo.

19 May 2016 7,326 1 View

How can I sequence my PCR product produced with the use of degenerate primers?

Can I simply add a random sequence to the 5' end of my PCR primers and use this as sequence primers? The degenerate primers are designed against a highly conserved part of G-alpha subunits and...

17 May 2016 1,935 5 View

How can I check for contamination in my RNA/cDNA sample?

I did a PCR reaction, cloning and sequencing on what is supposed to be dinoflagellate mRNA which I converted to cDNA. The resulting sequence is most similar to slime mold proteins. How can I check...

01 January 1970 4,846 3 View