21 February 2020 1 2K Report

Multiple-QTL Mapping in R/QTL

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How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

How can I increase transfection efficiency in my DC2.4 cell line?

I have been transfecting a dendritic cell line for about a year now with about a 15-30% transfection efficiency that I would like to increase to 40-50%. I have used Neon Transfection...

13 February 2024 8,526 4 View

Users or Past Users of Peyote and/or San Pedro?

I am a senior anthropology student at California State University Northridge (CSUN). I am looking for a few people who use/have used peyote/San Pedro for their health and/or religious/spiritual...

20 November 2023 6,616 0 View

How to prevent capillary clogging for zebrafish microinjections?

Hi all, I've been working on a project that involves microinjecting cells into zebrafish. A big issue has been needle clogging. Once capillaries are loaded, cells clog the tip of the needle and...

17 November 2023 8,670 4 View

How to reduce zebrafish mortality post injection?

I am doing an experiment involving microinjections with zebrafish and have been experiencing high amounts of mortality. The embryos look healthy upon injection, but many of them are dying between...

17 November 2023 5,510 2 View

Is transformation of agrobacteria with freeze-thaw method possible with large plasmid?

I have a large plasmid (20kb) which I am trying to transform into chemically competent commercial EHA101 cells. I had success with transforming a 16kb plasmid into the same strain, but have been...

07 August 2023 8,527 6 View

Can anyone recommend the best way to culture the Salmon Head Kidney (SHK-1) cell line?

Hello, I'm very new to cell culture, so I am trying to determine the best way to culture my cells. I have been trying to culture salmon head kidney (SHK-1) since March and it is growing...

06 June 2023 5,362 1 View

Protocol for BriteVu Perfusion of Mouse Coronary Vasculature?

I am trying to use BriteVu casting reagent to perfuse the coronary vasculature and image using microCT. I have tried 1) perfusion via catheterization of the descending aorta with ligation of...

11 May 2023 2,589 1 View

What theoretical framework is suggested?

What theory would you suggest in a dissertation with the title of: 'Teachers’ Perceptions of Efficacy in Integrating Social-Emotional Learning into the Curriculum for Middle School Gifted Students' ?

06 March 2023 2,927 3 View

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