21 February 2020 1 2K Report

Multiple-QTL Mapping in R/QTL

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Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to perform a mixed-effects Poisson regression with mutliple DVs?

I have a set of 6 related likert-type DVs, a within-subjects time variable (i.e. each DV was measured at 3 time points), and a categorical between-subjects IV (condition). Is there a way to...

16 December 2020 9,798 10 View

Repeated measures ANOVA with technical replicates?

I am doing an experiment where I measure the blood pressure of a treatment and control group over the course of several weeks. At each measurement session I collect multiple recordings. From what...

06 December 2020 531 7 View

Top-coded age and regression?

What is the best way to deal with top-coded age while doing regression analysis. If you are examining the presence or absence of a disease as your outcome, and a number of independent variables...

13 October 2020 907 5 View

Is there a guide that will help interpret Ponceau S staining for western blots?

Greetings, I'm having difficulty understanding my results from doing a ponceau S stain on a nitrocellulose membrane after western blot. I loaded 10ug in lanes 2,4, and 6. 15ug in lanes 3,5,and 7....

22 July 2020 4,616 1 View

Developing Calprotectin Assay for Bovines- extraction buffer selection and centrifuge settings?

Hello, I am trying to adapt the human Calprotectin assay to use in bovines. For background, Calpotectin is a protein released by neutrophils following gastrointestinal inflammation. A couple...

16 March 2020 1,261 3 View

Thickest sections possible for slide-mounted IHC of FFPE mouse brain tissue?

I am currently trying to section FFPE mouse brains using a rotary microtome for fluorescence IHC. I'd prefer to collect the sections free floating in buffer, stain them free floating and then...

04 February 2020 6,858 1 View

Antibody recommendation for IHC anti-IL18 staining in mouse brain free floating slices?

Hello, Many papers i look at include the santa cruz M-19 antibody which is now discontinued. I did find a few papers listing the R&D antibody raised against rat (AF521) but I believe this...

02 December 2019 8,578 3 View

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

18 August 2019 7,599 3 View

What can be inferred from ELISA OD values that are higher for wells tested with no capture antibody versus wells with capture antibody?

I am trying to develop and optimize my own indirect sandwhich ELISA by performing cherkerboard titration. I am currently checking different dilutions of the capture antibody against the antigen...

08 August 2019 4,926 7 View

Similar questions and discussions
What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

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