If the genes could be expressed, I can avoid transfer to another plasmid
Hello ! what kind of strains is it (the shuffle one ?) otherwise I am not sure that If they are not labeled as DE3 you will be able to do IPTG induction. This cell line is usually worth it for biofilm studies, not sure for expression ?
Cheers,
Recently there has been an upsurge in the number of predatory journals and publishers. These predators publish anything at all fee. Beall's list is a good starting point but what do scientists...
11 December 2017 9,363 5 View
We are a Kenyan Team working on traditional spontaneously fermented food products. Our project involves isolation and characterization of microorganisms using molecular diagnostic techniques and...
05 June 2015 9,146 4 View
I am currently working on a proposal on the application of lignocellulosic biomass for production of value-added chemicals in Kenya. I am looking forward to collaborate with a senior faculty...
11 December 2014 3,089 4 View
I want to detect the presence of fumonisins in some samples and later quantify the level of contamination. What is the most accurate yet simple method to achieve this?
03 April 2014 4,380 4 View
Polylactic acid synthesized by ROP may contain reminants of the catalyst. Who knows the minimum requirements of the catalyst for this polymer to be applied for medical uses?
08 September 2013 8,767 1 View
I have 3 plasmids; kanamycin, ampicillin and chloramphenicol- as markers and the plasmids are compartible. Can these plasmids be retained in E. coli without becoming a burden or should I transfer...
03 April 2013 8,438 5 View
I want to improve yields of my target compound and I believe by traditional metabolic engineering, I cannot easily target specific genes from the metabolic pathways.
02 March 2013 6,778 3 View
01 February 2013 1,813 11 View
02 March 2021 3,060 3 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?
23 February 2021 3,810 6 View