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Questions related from John Nduko
Recently there has been an upsurge in the number of predatory journals and publishers. These predators publish anything at all fee. Beall's list is a good starting point but what do scientists...
12 December 2017 9,421 5 View
We are a Kenyan Team working on traditional spontaneously fermented food products. Our project involves isolation and characterization of microorganisms using molecular diagnostic techniques and...
06 June 2015 9,207 4 View
I am currently working on a proposal on the application of lignocellulosic biomass for production of value-added chemicals in Kenya. I am looking forward to collaborate with a senior faculty...
12 December 2014 3,154 4 View
I want to detect the presence of fumonisins in some samples and later quantify the level of contamination. What is the most accurate yet simple method to achieve this?
04 April 2014 4,449 4 View
Polylactic acid synthesized by ROP may contain reminants of the catalyst. Who knows the minimum requirements of the catalyst for this polymer to be applied for medical uses?
09 September 2013 8,835 1 View
I have 3 plasmids; kanamycin, ampicillin and chloramphenicol- as markers and the plasmids are compartible. Can these plasmids be retained in E. coli without becoming a burden or should I transfer...
04 April 2013 8,500 5 View
I want to improve yields of my target compound and I believe by traditional metabolic engineering, I cannot easily target specific genes from the metabolic pathways.
03 March 2013 6,838 3 View
A plasmid with His-tag stored at -20°C for a year seems not to have the tag sequence, from sequencing and protein expression experiments. Has anyone had any experience with this?
02 February 2013 1,917 11 View
If the genes could be expressed, I can avoid transfer to another plasmid
01 January 2013 2,951 1 View
