How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

CAS solution whit PIPES ?

To prepare CAS solution for siderophores assay, the protocol use anhydrous piperazine. Can I make this solution using PIPES ? And how? Thanks The protocol i'm following is:...

08 November 2020 2,751 2 View

Bacterial siderophore production test/modified CAS assay results interpretation?

I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...

26 July 2020 7,194 4 View

Is this a indication of Siderophore producing bacteria?

Hi, I was testing for Siderophore producing bacteria but I found in one culture plate only a portion of my culture plate become pink in colour. What is the reason behind that ?

06 July 2019 7,122 9 View

Can we use Cu+2 instead of Fe+3 in Blue Agar CAS Assay for siderophore detection?

We are trying to find if our bactreia strains (Pseudomonas syringae pv. syringae) can produce siderophore that can acquire copper from the media. We used the classic assay of (Schwyn and...

31 December 2018 6,372 2 View

TLC plates fluorescent vs. non-fluorescent for bacterial siderophore detection?

What do you recommend for bacterial siderophore detection under UV light? Fluorescent or non-fluorescent thin-layer chromatography plates?

09 October 2018 3,520 0 View

Can I use CAS (Chrome Azural S) assay to measure siderophore production in iron containing media?

I just wonder that we can use CAS assay to determine the siderophore in culture media that contain some amount of iron or not ? According to the principle of CAS assay, the siderophore binds the...

06 July 2018 3,402 0 View

What are possible methods that could used for isolating the DNA of siderophores from fungi?

My partner and I are planning to conduct a study on siderophores found in fungi. We are wondering what are possible ways that we would be able to isolate its DNA.

01 February 2018 2,800 4 View

Are there ways that a siderophore could be physically isolated from fungi?

My partner and I are conducting a study in siderophores in fungi. We would like to know if there would be any way that we would be able to isolate the siderophore from the fungi itself.

01 February 2018 5,119 8 View

What are the different types of species of fungi that has siderophore?

My partner and I are conducting a research on fungal siderophores. We would like to know what species of fungi contains siderophores.

01 February 2018 5,440 8 View

Isolation of siderophore producing bacteria ?

Im working on isolation of siderophore producing bacteria from rhizosphere but im facing problem in blue color development when i add CAS dye in media

27 November 2017 7,872 5 View