The combination of reverse phase and open column chromatography is much needed for isolating active compounds from polar fractions. Can these resins be used in purification columns like that of the C18 columns, which on the other hand are not apt for open columns?
These resin (Diaion-HP-20, Amberlite-XAD-4,8,7,16 etc.) are quite good for preliminary purification of polar compounds like phenolics. When you will get your fraction after these resins then you can further purify your individual compounds in C-18 column and go for characterization.
Gud luck
:)
Using Amberlite XAD16 in an open-column chromatography, I was able... in just one round of elution, to isolate from my crude ethanolic plant extract a flavone. Purification was done by washing down the isolate with MeOH through a filter paper. Drying was done in a vacuum dessicator.
COSMOSIL 75C18-OPN or COSMOSIL 140C18-OPN are the packing material suitable for reversed Phase Open Column.
You can purchase Biotage C-18 packing material for open column.
BULK KP-C-18-HS form Biotage in 100g, 500g and 1kg packing. Its quite good and results are excellent. But cost is higher.
Gudluck
:)
Flow rate will be low. It requires a vaccume pump to elute the compounds from column.
100g app. 15 to 20000 Rs.
what about COSMOSIL 75C18-OPN or COSMOSIL 140C18-OPN
is it suitable for isolation of natural compounds and does it need a pump
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts