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Questions related from G. Balaji
Both 1H and C13 NMR values of my sample can be assigned to a known molecule except at one place. From reference values and theoretical point of view, a methine proton (CH) near to a methylene...
11 November 2016 2,878 5 View
The only reagent that I could use to see something is Blood reagent but the handling of this reagent is not as easy as anyother reagents. The fraction of such interest has been tried with spray...
08 August 2016 7,458 3 View
Kindly share your experience, if any, with bark phytochemistry. It would be grateful if I get a good tlc mobile phase for studying methanol extracts of bark that is known to contain polyphenols.
09 September 2015 9,686 0 View
I recently came by an article where toluene-ethanol mixture was used in soxhlet extraction. How efficient is this mixture of solvents than using single solvent for extraction?
06 June 2015 3,017 8 View
Please tell me if there is any in order to distinguish the spectra of terpenoids and steroids.
05 May 2015 9,739 9 View
I would like to know if there is any especially while purifying a molecule and also in obtaining NMR spectrum of thus purified molecule. Does the presence of trace DEA or its impurities while...
02 February 2015 4,196 5 View
Can vitamins be estimated/analyzed from dried samples preferably dried at 40 degree celsius?
02 February 2015 1,841 11 View
I would like to have one for my RP open columns. Please share your experience and thoughts. Also please share what kind of pump you have employed for your work.
01 January 2015 5,613 5 View
Looking for good explanatory notes to understand HMBC. Simple explanation with worked problem is also much appreciated.
12 December 2014 2,395 11 View
It would be very helpful for my work if anyone can suggest a way to remove perchloric acid from my sample for further chromatographic studies.
11 November 2014 9,658 3 View
What will happen to the yeasts if the vessel carrying out yeast fermentation is tightly sealed? Will the rising level of CO2 inside the vessel be toxic to the growing yeasts or the yeasts have a...
07 July 2014 5,725 8 View
I am estimating free and bound phenolics (after alkaline hydrolysis) using folin ciocalteu method. No problem with bound phenolics estimation as it gives blue color after incubation and the color...
07 July 2014 8,170 2 View
I have an alkaloid purified from plant origin but it is oily and very difficult to work with. I wonder whether making it to salt form would be better for my further work. Kindly share your...
07 July 2014 8,228 4 View
Can someone suggest a way to solve this problem? Is it a normal phase or a reverse phase the problem with sugar interference exists. My compound of interest in the extract is a water soluble one...
06 June 2014 7,809 3 View
Bound phenolic acids present in fruit requires extreme conditions in vitro to release them from a complex matrix. How does human physiology release and make them available for biological functions?
04 April 2014 4,036 3 View
I like to know the opportunities in terms of fellowships our govt offered for PhD students.
03 March 2014 8,124 0 View
I would like to know whether cells having both chloroplast and mitochondria organelles actively respire or the cells that lack chloroplast respire more.
03 March 2014 5,498 6 View
It would be good to have some insights on how this reagent reacts with nitrogenous compounds. Also what kind of nitrogen groups will react with this reagent? Because not all the nitrogen...
02 February 2014 904 12 View
When I heated glucose it turned as brown liquid. Is that brown color mean some degraded product of glucose? Could this be pyruvate as like that of end product of glycolysis during cellular...
01 January 2014 3,295 10 View
I got a good TLC separation for my polar fraction with only ACN-Water 9:1. Can I use the same solvent system with silica gel column for isolation? I did a search and ended up with a concept which...
01 January 2014 5,696 6 View
Initial fractions from Cosmosil column gave a precipitate which dissolves in MeOH or EtOH. But I cant find any spot on the TLC plate via Anisaldehyde-Sulfuric acid or Iodine vapour or Ceric...
11 November 2013 2,303 15 View
I would like to know about this column chromatography material in phytochemistry applications. Can anyone explain how to pack this material and how to load a sample which is non-polar. Also I'd...
09 September 2013 8,875 8 View
How far the use of diethylamine can be allowed or possible in purification of alkaloids by chromatography methods? Does diethylamine at any point of time react with alkaloids during purification...
07 July 2013 2,583 9 View
Got a water soluble fraction which is white and amorphous but no TLC detection by any means. So it is possible for an organic compound being low. Tried dissolving with acids and it dissolves well...
06 June 2013 7,761 8 View
Anyone with an experience of tannins isolation by column chromatography?
04 April 2013 911 3 View
I could see some oil in few of my hexane fractions. I wonder if there is any easier way to isolate this oil either by column or some other means.
02 February 2013 6,752 0 View
Can anyone suggest a silica gel (name a brand) exclusively for doing preparative TLC? I am facing additional spots after scraping-off the compound interest from the plates. Possibly due to...
02 February 2013 200 8 View
I am about to run my first C18 open column ever and would like to hear more about it. What is the procedure for packing the column? Can I pack the column with 100% methanol? Is pre-adsorption of...
12 December 2012 6,535 19 View
The combination of reverse phase and open column chromatography is much needed for isolating active compounds from polar fractions. Can these resins be used in purification columns like that of...
12 December 2012 6,180 9 View
It seems my compound of interest tightly bound to the Alumina column. Most of my impurities in the fraction had been eluted out but still no elution of my compound of interest even after 30%...
12 December 2012 4,702 2 View
I have isolated a pure compound from a plant source which could be either hydrocarbon or fatty acid ester as it is known from the IR and NMR analysis. How can I come to know about its volatile...
12 December 2012 9,047 17 View
I would like to install a solar panel in my home. I was told that 240W panel and 850W inverter would suit best for home needs. Since it is just 240 W how long it takes for charging this 850W...
12 December 2012 8,543 6 View
I would like to know where in India it is possible to do enantiomeric analysis either by GC or other means. Can anyone give me a suggestion?
11 November 2012 9,965 0 View
