I am a little surprised about the precise question here: Are you talking about matrices with real eigen values only? Or do you assume positive definite?
For hermitian matrices a lot of work was done in recent years, have to check the literature for this.
Also I'd like to see reference for the theorem (Weyl?) mentioned by Peter T Breuer.
Many thanks, however, I am still a little surprised. Assume we have two diagonal matrices A and B, then its obvious how the eigen values of tA + (1-t)B change. Now we take two commuting matrices A and B, then they are simultaneously diagonisable (provided both A and B are). So the same argument works as well.
Finally take A to be the identity matrix, then we get Weyl's theorem, since A is commuting with any B?
If A and B are not commuting, we have seen a counter example above.