Are houskeeping genes and essential genes preferentially located closer to the telomere or the centomere?

More Margaret Mchenney's questions See All

What are cultural bereavement practices of Jamaica that do not conflict with the Christian faith?

I am writing my thesis on the enculturation of Jamaican grief and bereavement practices that enhances their Christian faith and not diminish it.

23 July 2024 3,837 0 View

What is the best method to remove isolated CSF (mouse) from a micropipette?

We pulled our own micropipettes to extract CSF from anesthetized mice. We do not use a micrometer/syringe system, we just gently poke the micropipette tip into the appropriate location to isolate...

13 May 2024 2,632 0 View

research on simultaneous interpreters and executive functions?

It's been recommended that I look into the Inhibitory function, but I welcome any suggestions. I am a credentialed interpreter (US Court certified) and I'm curious about any and all functions that...

11 March 2024 8,157 0 View

How did the researchers systematically analyze the influence of defined preanalytical conditions on LD, potassium and hemolysis index in vacuum blood?

According to a study by Nick Neuwinger et al. (October 2019) on the impact of underfilling vacuum blood collection tubes on lactate dehydrogenase activity, systematic approaches were used to...

20 February 2024 2,166 1 View

Factors influencing consumer perception regarding cosmetics, questionnaires?

i need to find an existing study with survey questions

20 January 2024 2,571 0 View

Differentiate between self-care and self-management?

These two words are often used interchangeably, some even used the same definition for both. However, they are no the same.

19 December 2023 4,644 19 View

Can anyone make any recommendations on literature centered around whole systems change and cultural impacts, values-based leadership or ethics?

I am hoping to find good, peer-researched literature that assists me in developing a collection of good data on this subject.

01 November 2023 6,239 2 View

Has anyone used a pDARPP-32 antibody for immunohistochemistry?

I want to use a pDARPP-32 antibody for immunohistochemistry to stain for phosphorylated DARPP-32 in specific brain regions. Does anyone have experience using a pDARPP-32 antibody for...

10 October 2023 8,095 0 View

What are the best approaches for multi-lingual learners in co-requisite education?

How do ESL students learn best in higher education in terms of reading and writing?

30 August 2023 8,287 2 View

What is an antibody for DARPP-32?

Does anyone know of a good antibody for immunohistochemistry to stain for DARPP-32? Or does anyone know of an immunohistochemistry antibody to stain for D1 receptors? Or an alternative...

09 August 2023 8,159 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View