What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

I am staining mice muscle for macrophages (CD68) and lymphocytes (CD3). The challenge that am facing is that my control is also staining with the secondary antibody even though I did not add the...

15 October 2020 8,139 3 View

Triangulation of data sources in qualitative research: Do you consider it mixed methods research and why, whether its a yes or no?

It is common in qualitative research to interview for example patients and their partners or their health professionals to talk about the same topic and explore the perspectives of all the people...

30 July 2020 538 21 View

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house ?

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house that is a step down from inpatient unit. The study will look at...

12 May 2020 7,072 3 View

Who can link me to Cost / Benefit Analysis of Treatment Options for Produced Water of varying quality and quantity?

I work in the oil industry and I wish to focus on reducing reinjection of "free" (non-water right controlled) produced water for beneficial use where economics dictate feasibility. Who can direct...

24 February 2020 3,059 2 View

How is confirmatory factor analysis not just a tautology?

Hello, very new to confirmatory factor analysis (CFA) and structural equation modeling, and I'm trying to understand how to think about confirmatory factor analysis. Suppose I have a construct,...

11 November 2019 3,330 4 View

How to deal with member check conflicts in qualitative research?

Member checking or participant checking is done to validate and improve the credibility of quallitative research results. I am planning to ask my participants to help with that (will ask them to...

27 May 2019 7,799 13 View

How do you create a calibration curve for H2O2 ?

Specifically I'll be looking at H2O2-titanium complexes (in plant tissue) and will be measured @ 415nm. I'm wondering how I can create a calibration curve for the complex... I have 30% H2O2 and...

13 December 2018 4,432 3 View

Improvised abacus? Are there Latin references to betting at Roman amphitheatres, or modern references to Roman use of abaci? Or was it a gaming board?

11 June 2015 1,010 68 View

Do we have instrument development experts among rehabilitation professionals in Sub-Saharan Africa?

It has been noted that clinicians in Africa are forced to use instrument measures that were developed and validated either for American and European children which are quite limited. It is...

01 January 1970 528 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View