How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

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26 December 2020 9,337 3 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

How to solve MSTRAT software Running problem?

Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error: ether couldn't execute...

25 November 2020 9,748 2 View

How can I classify Brain Waves on Python?

I'm working in my thesis project that's about how to avoid car accidents by body fatigue, I got some datasets where I got information abouth 12 person that had an EEG analisis when they were...

07 November 2020 4,845 2 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

What books on anticancer potential of polysaccharides obtained from fungi recommend me to read?

I am investigating the medicinal potential of the polysaccharides present in edible fungi native to my country and that can help prevent or treat some types of cancer.

16 February 2020 7,775 3 View

Any behavior test for social novelty apart from 3 chamber test?

Any behavior test for social novelty apart from 3 chamber test? I want to look if there is an impairment in social novelty or social memory (in rats). I wanted to know if apart from the 3 chamber...

11 November 2019 9,876 3 View

How to cite chinese names?

This may look like an odd question, but since I'm latinamerican I'm not familiar with chinese names, the usual way to reference names is by writting the last name and the initial of the names, but...

11 September 2019 9,386 3 View

Does anyone knows this plant?

I need samples of a plant named Kermadecia sinuata, according to the data I got it is found in New Caledonia, anyone knows any repository of exotic species that could have this specimen and that...

12 February 2019 2,125 2 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What is difference between In-line transmission (%) and Total transmission (%) value of optically transparent Ceramics?

I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...

20 February 2021 9,405 6 View

In your experience what is the difference between G.652D and G.655D optical fiber. In field and in lab?

In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...

11 February 2021 4,006 8 View

I am looking for a microbend optical sensor design...can anyone please provide me some idea about it??

I am working on a landslide model...i need an optical sensor to determine the amount of pore pressure and other values..i am thinking of using a microbend sensor. Can anyone please provide me with...

11 February 2021 5,911 2 View

Feature Tracking in Python?

I have been trying to track feature points in the video using opencv. However, use of Shi Tomasi Corner Detection along with optical flow algorithm like Lucas Kanade Optical Flow doesn't seem to...

09 February 2021 8,475 3 View

Is 60%/40% a typical fiber-matrix ratio in vehicle GFRP/CFRP composites?

Quick question I couldn't easily find a definite answer, so I hope someone here could help. I've been looking for information about the ratio (by weight) of glass or carbon fiber to epoxy matrix...

04 February 2021 740 1 View

What is the reason of the received optical signal to be slimmed down in multi-mode fiber?

What is the reason of the received optical signal to be slimmed down in multi-mode fiber? I have 4 km multi-mode fiber

03 February 2021 1,105 10 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Why it is not permissible to correct all 5 classical optical aberration (spherical, chromatic, coma, astigmatism, curvature of field) simultaneously?

When first order paraxial optics is expanded to third order optics (i.e. sin x~ x-x3/3! instead of sin x~x) , these 5 optical aberrations result in. What is the rigorous mathematical proof that,...

26 January 2021 8,666 4 View