I am interesting if there are any databases where would be possible to find whole scientific projects? with summary, aims etc. like NIH Crisp reporter, but there are only summaries.
I used a 12-well plate, and there were 1.5x10^5 NIH-3T3 cells in each well. After 20 hours, when I used a pipette tip to create a scratch and changed the medium, a portion of cells at the well...
26 February 2024 4,903 0 View
I am working on a 3T3L1 cell line. I received my cell line with a passage number of 7. Cells were attached until the passage number reached 10. However, after that, cells began detaching from the...
11 February 2024 4,086 0 View
i have 100NIH units of thrombin and its molar mass is 37.4kDa, how to make the solution and how do determine it's molarity?
27 July 2023 6,359 0 View
Hello! I'm trying to understand if the experimental question on which I would like to work on has already been chosen as the centre of interest of some other big research project, and I don't...
21 July 2023 6,826 2 View
I am currently differentiating 3T3-L1 cells and I am uncertain about the presence of lipid droplets. However, before conducting the Oil Red O staining, I would like to know if there are already...
27 June 2023 406 4 View
Hi there, we are trying to establish stable transfected NIH 3T3 or C2C12 cells by FACS sorting (on plastic dishes), and starting off with 90 % green cells after the sort, we end up with hardly...
08 December 2022 1,674 4 View
Hello, which tool would you suggest for assess of RoB of the uncontrolled interventional before-after studies. There are such kinds of clinical trials, in which the participants are checked before...
14 October 2022 137 2 View
If am proceeding an experiment and I want to follow the Euthanasia of Lab Animals after finishing my experiment. Is there a Libyan guideline for that or I should follow the AVMA Guideline or NIH ?
03 August 2022 9,797 0 View
No affiliated organisation to attach.
22 March 2022 3,442 1 View
I am attempting to induce senescence in NIH3T3 cells to treat with compounds to test their hypothesized anti-senescence activity. However, I have been having some challenges. Several reviews and...
16 February 2022 6,118 2 View