About loading of Doxorobicin on MOF (drug delivery)?

Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...

07 February 2021 748 2 View

How can I represent a 2-parameters function in series type?

I want to represent a function like f(x,y)=sin(1/(x+y)) about [x,y]=[0,0] in series type. how can i do it in matlab or maple?

10 January 2021 8,389 5 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

Challenges in growing deep learning model?

I implemented an image classifier and object detection model. I added a new class every day to my model and the data set growing too. I wanted to ask if anyone has the same experience? at now it...

03 January 2021 4,652 5 View

Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

What causes different microstructure in a sample?

I have synthesized high entropy oxide with (Ni Zn Cu Mn)1-x Cox composition. At x=0.05 the microstructure became rod shape and the SEM picture of the other part of that sample is different. And...

12 October 2020 5,576 5 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

Antibiotic screening of drug resistance cancer cell line with MDR pumps?

Hi. during our study, we faced with a problem in the screening of EPG RDB 257 cell line which is a resistant cell line due to the expression of efflux pumps such as MDR 1 which actively excrete...

27 August 2020 9,192 1 View

How to calculate forces acting on Large industrial centrifugal fan blade to be considered in the Finite Element modelling?

hi friends. i m working on large industrial centrifugal fan to investigate causes of failure. How to calculate forces acting on Large industrial centrifugal fan blade to be considered in the...

12 August 2020 2,769 9 View

Why dose force become constant in two way FSI?

Dear friends I'm working on the validation of an FIV case, which for making phase difference between force and displacement, I used an initial velocity in the structural, but after several...

10 August 2020 9,057 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View