Artificial refuge for reptiles?

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Little to no signal with BONCAT and SPAAC protocol on environmental marine bacterial samples?

Hi, I have been developing a BONCAT protocol for use on environmental marine bacterial samples using L-azidohomoalanine (AHA). From my testing and literature research, I found that incubating...

14 November 2021 7,039 0 View

Can I cite papers I don't have access to?

Many papers are behind a paywall and I can't read them without using tools like SciHub (even my institution login doesn't give access to many papers). I would like to cite some of these papers and...

16 June 2021 3,601 43 View

Any journal articles on pesticide sorption/desorption, dissipation, movement specifically in saline/sodic soils?

References for saline/sodic soil and pesticide interactions (sorption/desorption, movement, 1/2 life, etc.)

15 June 2021 4,625 3 View

How to reduce bacterial load in an algal culture?

Hello, I was wondering if there were any methods commonly used to reduce bacterial load in an algal culture. These aren't axenic cultures but the microbial load is a little high, since we'd...

30 September 2020 7,405 4 View

Ardales is reported as having a clay surface over rock being engraved, covered in calcite then eroded (also fluting) do you have more details of this?

I am focussing on clay in the Palaeolithic for my dissertation and reports on clay in Ardales are vague, including non-representative finger fluting and evidence for clay over a block being...

26 August 2019 9,583 0 View

How do I maintain a consistent monolayer in an organotypic skin culture?

I have been attempting to recreate an organotypic skin culture using primary fibroblasts and primary keratinocytes. I start by embedding fibroblasts in a collagen gel at 200,000-300,000 cells/mL....

18 June 2018 6,461 6 View

What could cause my DNA bands to run too high on my electrophoresis gels while using sodium borate buffer?

I just switched over to using a sodium borate buffer for my DNA electrophoresis instead of TBE. I did this because SB is faster, cooler, and cheaper than TBE or TAE. The problem is that my DNA...

06 September 2016 7,631 2 View

During the RNA precipitation step with isopropanol during a TriZol extraction, can glycogen carry contaminants into the RNA pellet?

I have been doing TriZol RNA extractions of zebrafish embryos with and without glycogen as a carrier. I've found that using glycogen during the isopropanol precipitation step increases the size...

15 August 2016 6,882 6 View

How can I prove hydrogen bonding with FT-IR?

I have an amide-containing compound, and I would like to show that it is undergoing hydrogen bonding with a polar aprotic solvent. Can this be done with FT-IR?

18 July 2015 6,788 7 View

What is the best way to cure PDMS (to be bonded via O-plasma for microfluidics)?

I am looking for a protocol of making PDMS that is optimal for bonding it to PDMS or glass after RIE in my oxygen plasma chamber. I'm aware of the "in-box" curing protocol.

15 June 2015 4,809 4 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

What are examples of AI for good projects a teacher can assign to students?

So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?

08 August 2024 9,437 4 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

How to design human-centered classroom in the age of A.I.?

08 August 2024 347 5 View

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

What's the role of IT & AI in Telecommunication Industry?

05 August 2024 8,264 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

James Des Lauriers

Hello Clay; It sounds like you have something specific in mind. It might help your readers if you tell a little story about whatever that is. That would allow the reader to focus on what you are interested in. Best regards, Jim Des Lauriers

Adityas Arifianto

Dear Simpkins, this article maybe can help :

https://www.marwell.org.uk/wp-content/uploads/2021/05/hardy_s._2020.pdf