Can anybody suggest methods of induction of aplastic anaemia in rats?
Long-term exposure to benzene vapors is associated with hematological diseases such as leukemia, lymphoma and aplastic anemia. here is a paper that may help.
http://www.sciencedirect.com/science/article/pii/S0300483X01003717
Yes benzene is the best one, particularly in mouse.........If you go back in literature articles of Robert Snyder, Bernard Goldstein, David Eastmond & Martyn Smith, you can find a lot about benzene induced aplastic anemia. Interestingly David and Martyn published in early 80's that combination of phenol and hydroquinone administration to mice can reproduce benzene induced aplastic anemia....You can induce aplastic anemia with one or two acute doses of benzene or phenol/hydroquinone combinations....Hope this helps
In animal models of mice and rats, benzene only causes aplastic anemia but not leukemia or lymphoma, even upon chronic exposures mimicking humans. There is no rodent model for benzene induced leukemias. In humans benzene induces leukemias, lymphomas and aplastic anemias.
Aplastic anemia is characterized by decreased number of all blood cell types, whereas leukemias and lymphomas are characterized by increased number of particular blood cell types. Some scientists believe that aplastic anemia is a preleukemic condition but there is no evidence supporting that hypothesis.
What is the preferred route of administration of benzene to induce aplastic anaemia in wistar rats?
Usually mice are best. you can use sc route, which is the best
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