Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

How to remove rotation along z-axis and shift the origin to body's center?

I have attached the files with this post to explain me how to remove rotation and center the origin at the body's local coordinate. This is just one sample and I have 1000s of files like this. I...

18 December 2020 3,838 3 View

How can we know the exact soil taxonomy of any particular site. Is there any online available database for Indian soil taxonomy based on geography?

Please If one can let know the soil taxonomy for vindhyan region falling in Mirzapur district of Uttar Pradesh, India.

14 December 2020 9,879 14 View

Does anyone knows a tool (GOMS based or other) to automate calculation of task completion time?

Hello good human, really hope that you are doing well. I have been reading "The Psychology of Human Computer Interaction" by Stuart Card et al. They have introduced one of the ground breaking...

11 December 2020 7,269 3 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

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30 November 2020 5,089 5 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

How can an Undergraduate collaborate with Professors globally for research projects?

I am seeking to increase my experience by working on research projects and aiming to present them as papers

01 February 2021 3,800 4 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View