20 November 2019 0 1K Report

Hi Everone!

Anybody working on drug resistant breast cancer cell lines in TEXAS, USA then please let me know.

thank you

More Shumaila Zaib's questions See All
seeking opportunities PhD in Public Health Program 2024 Intake?

As a dedicated researcher and professional in the field of mental and public health, I am enthusiastically seeking opportunities to pursue a Doctorate degree in various disciplines. My interests...

14 April 2024 8,094 2 View

Railway track GROSS MILLION TONS CALCULATION METHOD considering multilple factors such as speed ,axle load etc for pasenger and frieght trains ?

Railway track GROSS MILLION TONS CALCULATION METHOD considering many factors such as speed ,axle load etc for pasenger and freight trains ? need help and not the thumb rules

12 December 2023 2,267 3 View

How do you calculate the limit of detection (LOD) of an electrochemical sensor?

How can I calculate the LOD of an electrochemical sensor used for the detection of H2O2? I am aware of the formula LOD = 3Sb/s, where Sb represents the standard deviation of the blank solution and...

04 July 2023 5,049 1 View

How do write a research Proposal ?

Issues and challenges in NER.

26 June 2023 3,311 2 View

How to set Bio-RAD CFX connect for qPCR using TaqMan Probes/primers?

Our lab instrument for qPCR is BIo-RAD CFX connect with CFX maestro program installed in it. Previously our system was set on SYBR. Recently, I started working on TaqMAn 1: TaqMan FAst advanced...

26 April 2023 9,363 1 View

What's the comparison of strength between flash butt welding and thermite welding ?

What's the campaign of strength of flash butt welding and thermite welding ? Any research please share .

09 February 2022 4,692 1 View

Effect of amalgamation of coaches having different braking and suspension system in train on derailment ?

Effect of amalgamation of coaches having different braking and suspension system in train on derailment ?

24 January 2022 2,944 1 View

Whats Comparison between RN rail fastening and new type of rail fastening e.g w-14 etc ?

Is there any literature elaborating the performance and effectiveness of RN fastening , performance of it else any comparison between the RN fastening with new type fastening in rail...

25 November 2021 9,361 3 View

How to select rail cross section for railway line ?

What is the latest way to design /select the rail cross section/profile Keeping the maximum axle load and traffic daily tonnage in consideration or any other criteria . Please any guidance or...

01 August 2021 5,797 3 View

What is railway track damage due to higher axle load and speed ?

what will be the damage to due speed of higher axle loads in case of fatigued rails ?and track design being nearly equal to axle load e.g sleepers have been designed for 22.8 tons and axle load...

20 June 2021 8,310 2 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close