Hello Suman, Thank you for your interesting question. IR spectroscopy can be used to perform quantitative evaluations in tag. However, for this purpose, the spectra must be recorded in absorbance (Abs.) mode, not in transmission mode. By integrating the peak area, quantification can then be performed using a calibration series.

See also: https://www.researchgate.net/post/How_can_i_use_FT-IR_spectroscopy_in_quantitative_analysis

Best wishes, Florian

Please look at the following below links which may help you in your analysis:

https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-017-0817-3.pdf

https://www.tandfonline.com/doi/pdf/10.1080/10942912.2012.692224?needAccess=true

https://www.scielo.br/j/rmat/a/NnvbLg5J7W3fVX6XPvK94QL/?lang=en&format=pdf

Thanks

Yes! I did it for silicone oil. However, remember low energy (like FTIR) means low response. You will be lucky to have an LOQ of 100 ppm or mg/L. Mine was 25 ppm.

Take a look at these.

1. Baker, M.J., et al., Developing and understanding biofluid vibrational spectroscopy: a critical review.Chemical Society Reviews, 2016. 45(7): p. 1803-1818.

2. Durlik-Popińska, K., et al., Correlations between autoantibodies and the ATR-FTIR spectra of sera from rheumatoid arthritis patients. Scientific Reports, 2021. 11(1).

3. Fang, T., et al., Rapid Diagnosis of Type II Diabetes Using Fourier Transform Mid-Infrared Attenuated Total Reflection Spectroscopy Combined with Support Vector Machine. Analytical Letters, 2017: p. null-null.

4. Finlayson, D., C. Rinaldi, and M.J. Baker, Is Infrared Spectroscopy Ready for the Clinic? Analytical Chemistry, 2019. 91(19): p. 12117-12128.

5. Rüther, A., et al., Vibrational Spectroscopic Based Approach for Diagnosing Babesia bovis Infection. Analytical Chemistry, 2020.

6. Sharaha, U., et al., Using Infrared Spectroscopy and Multivariate Analysis to Detect Antibiotics’ Resistant Escherichia coli Bacteria. Analytical Chemistry, 2017.

7. Banerjee, A., et al., Rapid Classification of COVID-19 Severity by ATR-FTIR Spectroscopy of Plasma Samples. Analytical Chemistry, 2021.

8. Nogueira, M.S., et al., Rapid diagnosis of COVID-19 using FT-IR ATR spectroscopy and machine learning. Scientific Reports, 2021. 11(1): p. 15409.

9. Pokhrel, P., C. Hu, and H. Mao, Detecting the Coronavirus (COVID-19).ACS Sensors, 2020. 5(8): p. 2283-2296.